Apart from the above-mentioned diseases, the glycoprotein (G) of spring viremia of carp virus (SVCV) and ORF81 protein of koi herpesvirus (KHV) have been proved to be functional antigens and co-expressed on the surface ofL. plantarumHA33-1 to provide protective immunity for cyprinid fish [...
The EPC cell line was genetically engineered to overexpress a monomeric, cytosolic form of EGFP (mEGFP) and the nuclear nCas9n using the same method used to develop the CHSE-EC cell line [44]. Briefly, the EPC cell line was engineered using the plasmid pcDNA3.1-Hyg-nCas9n (Addgene #21...
To characterize further the diversity of the finTRIMs, we performed a 3'RACE PCR on VHSV-induced leukocyte cDNA using a universal primer specific for trout finTRIM localized in the highly conserved region in the vicinity of the start codon. These experiments on infected leukocytes revealed a rich...
The Southern Ocean (SO) has undergone a series of large episodic glacial-interglacial changes, with cycles that have lasted about 100,000 years during the latter part of the Quaternary Period (the past one million years) [1]. These environmental changes have impacted the speciation events in th...
[2–5]. They are usually encoded as a single exon, and together form the 'RBB' region. In addition, the RING finger has E3 ubiquitin ligase activity [6]. The coiled-coil region seems to be predominantly necessary for multimerization, resulting in the formation of high-molecular weight ...