As a prevailing form of chronic liver disease, nonalcoholic fatty liver disease (NAFLD) affects approximately one-fourth of the global population [1]. The incidence of NAFLD has continued to increase in parallel with the prevalence of type 2 diabetes and obesity [2]. NAFLD includes simple liver...
Because IRE1 and PERK are functionally active in a phosphorylated form, the corresponding phosphorylated forms were also evaluated. The results showed an increase in each of these ER stress-related proteins in SelK knockdown of LN229 and U251 cells compared to the control group (Fig. 8C). ...
qRT-PCR: Real-time quantitative reverse transcription polymerase chain reaction RH: Ringer-Hepes buffer RT: Room temperature RTK: Receptor tyrosine kinase scCM: Super-concentrated conditioned medium SP: Stroke patient TIMP1/4: Tissue inhibitor of metalloproteinase 1 and 4 TJ: Tight junction...
qRT-PCR analysis was used to validate gene modulation. Results Pre-CPB samples showed significant differential expression of a total of 72 genes, 28 of which were overexpressed in ToF and 44 in ASD. According to Gene Ontology annotation, the mostly enriched biological processes were represented by...
Bone tissue regeneration presents a novel approach wherein externally constructed tissues replace damaged or missing tissues to restore the form and function of deficient bone tissue in patients [1]. Recent advancements in Bone Tissue Engineering (BTE) technology are poised to effectively address ...
Adipose-derived stem cells (ADSCs), which are MSCs that are present in adipose tissue, are abundant in the body and are easily clinically transformed; thus, these cells have great potential in the field of tissue regeneration. Previous studies have shown that intra-articular injection of MSCs ...
[182]. Additionally, monoclonal antibodies in the form of the synthetic single-chain variable fragment (scFv) isolated from antibody phage display are currently being developed and current data demonstrated that the scFv targeting AGR2 was able to bind the N-terminal of AGR2 with high affinity [...
Methods commonly used for RNA virus detection include RT–PCR, real-time PCR (qRT–PCR), PCR cloning for Sanger sequencing and in situ hybridization [15]. However, traditional detection methods are limited in that the genome sequence of the virus has to be known before detection. In contrast...
Screening of hub SE-associated gene IGF2BP2 in HNSCC Cancer cells commonly form oncogenic SEs on crucial oncogenes, which drive oncogene transcription and play a vital role in the initiation and maintenance of cancer characteristics. To explore the significance of SE alterations in the tumorigenesis...
Although all cell lines could form tumors at a density of 1 × 106 cells, STARD13-correlated ceRNAs-3′UTR-overexpressed cells showed a decrease of tumor size and weight (Additional file 7: Figure S3A and Fig. 2a). Notably, the tumor-seeding ability of STARD13-correlated ceRNAs-3′...