The PCR conditions were as follows: 95 ℃ for 5 min for 1 stage, 95 ℃ for 10 s, and 60 ℃ for 40 cycles. The samples were normalized to GAPDH. The sequences of the primers used in this study are listed in Supplementary Table 2. Multiplex Immunohistochemical (mIHC) Multiplex ...
The use of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) in cancer therapy has the potential to revolutionize the way for treating different diseases [1]. CRISPR technology allows for precise and efficient manipulation of the genome, and its application in cancer research has the ...
The gold standard for the diagnosis of COVID-19 is by RT-PCR of nasal or throat swabs. The first positive test was set as the date of diagnosis, and the date of two consecutive negative PCR tests was used as the cutoff, to calculate the length of the hospital stay. Mortality during ...
Understanding the mechanistic effects of novel immunotherapy agents is critical to improving their successful clinical translation. These effects need to be studied in preclinical models that maintain the heterogenous tumor microenvironment (TME) and dys
A diagram of the sample number of diarrhea virus detection results by real-time PCR. The number of samples that were successful in typing by PCR and Sanger sequencing was indicated in the square brackets [ ] Full size image Of the 93 patients in this study, 46 were male, and 47 were fe...
To confirm the causative agent, fecal swabs were delivered to our lab and screened for enteric virus using RT-PCR assay. The test result showed that only the nucleic acid of PDCoV was positive, and that of the PEDV, TGEV, PoRV and SADS-CoV were not detected (Fig. 1B). Although farm ...
S1B). Long-range PCR confirmed the correct targeting of 5′ and 3′ homolog arms (Supplemental Fig. S1C). To test the effectiveness of our cKO strategy, fibroblast cells from homozygous Rbm8afl/fl mice were infected with an RFP-cre virus in vitro. RBM8A protein was absent in RFP-...
CRISPR/Cas9-mediated targeted inactivation of p53 and Pten in mouse liver induced intrahepatic cholangiocarcinoma (ICC), a second form of primary liver cancer, in mice [19]. Multiplexing CRISPR/Cas9 has also been explored for high-throughput analysis of TSGs for initiation and development of liver...
encoding VSVg, two packaging plasmids encoding rev, gag and pol genes, and a transfer DNA plasmid vector, was added to pre-warmed OptiMEM (Thermo Fisher Scientific, 31985070), followed by addition of PEIpro and incubation for 30 min at room temperature for the transfection complexes to form....
2g). Similarly, full-length coverage with Smart-seq3xpress resulted in significantly (2–5-fold) increased read support over exon–exon and exon–intron splice junctions, which form the basis for RNA velocity inference16 (Fig. 2h). To perform a direct comparison of cluster granularity, we ...