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The four gRNAs were implemented as a CRISPR-array, along with the tracrRNA and Cas9-components and incorporated into a single vector containing all parts necessary to form the fully functional curing system designated “pFREE” (Fig. 2b). An important feature of a plasmid-curing system is a ...
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For the statistical analysis of the trials, two linear mixed models were used to take account of the diversity of the experimental design. The general form of the base models was: y=Xb+Zu+e where y is the vector of phenotypes, b is the vector of fixed effects, and X is the associated...
Testing of these traits on ellipsoids drawn at different pixel resolutions found the volume estimation to be within 0.05% of the true value and the surface area approximation to be within 5% of the standard approximation (as the surface area of an ellipsoid has no closed-form solution). We ...
The Growth Value Model (GVM) proposed theoretical closed form formulas consisting of Return on Equity (ROE) and the Price-to-Book value ratio (P/B) for fair stock prices and expected rates of return. Although regression analysis can be employed to verify these theoretical closed form formulas,...
The data from the TUG were reciprocated, to have them in a similar form to the data of the 10MWT, which are expressed as speed (m/s). This allows the TUG data of patients who could not perform the test to be expressed as numeric value (0 s−1). Results Robotic variables that ...
in addition to exploring outdoor cultivation technology to maximize the production of biomass, a high-value-added substance [30]. The culture parameters include bioreactor type/form, gas supply method (e.g., the composition and concentration of a gas and flow rate), pH, temperature, light inte...
In these data, the most determining factor for performance was a form of deliberate practice, namely training with the general explicit goal of typing fast. The number of keystrokes accumulated since the beginning of practice (i.e. the actual typing experience) also affected performance. In ...