One study showed that paralogs are less likely to be essential in whole-genome CRISPR knockout fitness screens than singleton genes [15], while another study demonstrated that some paralogs that form heterodimers are more deleterious to the cell compared to non-heterodimer forming paralogs [16]. ...
The species causing the most severe form of the disease is Plasmodium falciparum (Box 1). Although insecticide-treated bed nets, and other preventive measures are important in the control of malaria, in the absence of a licensed vaccine and acquired, fully-protective immunity, chemotherapy has ...
Negative controls in the form of a PCR-amplified ultrapure water sample were included for each batch. PCR products were visualised on an agarose gel: bands were visible for all 40°Cycle samples and the first four dilutions of the 20°Cycle samples. Data are deposited under ENA project ...
Purification tags used today are classified into peptide-tags and protein-tags based on their nature and form. His-tag, a typical and globally the most used peptide-tag, is highly effective in purifying the tagged protein by using immobilized metal affinity chromatography, such as nickel sepharose...
[22] performed genomic analysis on 28,576 sequences available in GISAID database (by May 29th, 2020) and shown that a SARS-CoV-2 variant carrying the spike protein amino acid change D614G had replaced the original Wuhan form of the virus across the globe by June 2020. A variant linked ...
As a result, the activated ECs increase the production of angiocrine factors that confer many growth advantages to tumor cells. The angiocrine factors may be in the form of transmembrane ligands, secretory cytokines, or microparticle-packaged factors. There is evidence that activated ECs trough their...
Kit (Tiangen Biotech) according to the manufacturer's protocol. The viral genome was reverse transcribed using 200 units of M-MLV (Promega) in a 20 μl reaction volume with the anchored random primer (5'-GCCGGAGCTCTGCAGAATTCNNNNNN-3') at 37°C for 1 h to generate a viral cDNA pool....
FITS (Flexible Image Transport System) is a binary format of many astronomical datasets and images. The library requires a level of familiarity with FITS and its common standards and conventions for effective use. For example, while the library will automatically interpret and populate the mandatory...
coding RNAs and may have regulatory roles in gene expression. Strand-specific transcriptome sequencing has led to the description of massive amounts of antisense RNAs in various bacteria, eukaryotes, and archaea, and might represent a common form of regulation within all domains of life [22,91]....
M. domestica Teps are labeled XP_NNNNNNNNN and are shown in italics. For both species, multiple isoforms of the same gene are indicated with a bar. Branch support is the SH-like statistic estimated in phyml. The tree is rooted so as to minimize the number of duplications. Full size ...