Optimization of cultural conditions significantly enhances the yield of cellulase enzyme when compared to unoptimized culturing conditions. Additionally, heterologous expression of egl gene showed that the recombinant form of the cellulase is active and that a valid expression system can contribute to a be...
Gene fusion refers to a sequence in which some or all of the sequences of two genes are fused through some mechanism (such as genome variation) to form a new gene [50, 51]. A fusion gene can induce the production of SEs, thereby driving the transcription of oncogenes. Benbarche et a...
We used overexpression and knocking down lentivirus to clarify the influence of HDAC5 and HDAC6 in melanoma development. Also, we introduced stable HDAC5 or HDAC6 knockdown cells into null mice and found that the knockdown cells were unable to form solid tumors. Finally, we tested HDAC5 and ...
Class I HDACs work in the form of multiprotein complexes, such as NuRD, Sin3, and CoREST, and are implicated in various physiological processes, including transcriptional repression, chromatin assembly, cell cycle progression, and maintenance of genomic stability [12]. Class II HDACs, including ...
More than 10% of cell surface proteins and the majority of transmembrane proteins require proteolytic digestion to release a soluble form for activity [16]. Proteolytic cleavage modulates cellular signals and affects cellular homeostasis depending on the pathway in which the substrates or receptors are...
Inhibitors of HDACs include trichostatin A (TSA), nicotinamide (NAM), suberoylanilide hydroxamic acid (SAHA), and LBH589 Full size image Lysine crotonyltranferase (writer), decrotonylase (eraser), and reader The modulators of histone crotonylation have been extensively studied and summarized earlier...
Imaging examination: a and b showed diffuse interlobular septum thickening in both lungs to form a grid opacity, thickening of bronchial wall, and consolidation in the left sublobal lung. c showed diffused grid-like opacities in both lungs, especially in the left lung Full size image (2) ...
Humans also display a centromeric copy of the gene, SMN2; it differs from the SMN1 copy for a C to T transition in exon 7 that results in splicing defects and that in most cases leads to the production of a truncated, unstable and not functional form of the protein, called SMNΔ7 [...
Several new therapeutic strategies are now considered for acute myeloid leukemia (AML) patients unfit for intensive chemotherapy, including modulation of protein lysine acetylation through inhibition of histone deacetylases (HDACs). These enzymes alter the acetylation of several proteins, including histones ...
DNA wraps around an octameric complex of core histones H2A, H2B, H3 and H4 to form nucleosomes. DSBs induced by ROS, replication stress, or by exogenous agents like UV, radiation, radiotherapy, or other genotoxic agents are thought to be the most dangerous lesions for genomic integrity [33]...