have shown that when cells are stressed by treatment with thapsigargin or high extracellular concentrations of KCl, clusterin is retained in the cytosol instead of being secreted45. In our study, both chains di
These cells are only able to form one cell type, e.g. dermatocytes. Stem cell biology A blastocyst is formed after the fusion of sperm and ovum fertilization. Its inner wall is lined with short-lived stem cells, namely, embryonic stem cells. Blastocysts are composed of two distinct cell...
The development of the cortex is a delicate balance between proliferation, differentiation, and migration of neural progenitors (NPs). Throughout developmental process, various cellular mechanisms ensure that NPs differentiate into the correct cell subtypes, migrate to their correct regions, and form the...
This is indicative of the importance of the five residues of the B chain C-terminal in the insulin misfolding process. Overall, simulating full-length insulin under high temperature and in the presence of KCl could be used to assess amyloid formation and potential amyloid inhibitors of this ...
[416]. The release of this truncated form of Tau is increased upon synaptic activity [216]. However, a study investigating the level of truncation in AD patient brains has shown that there is a relative increase in N-terminal truncations of Tau in AD patients as compared to controls [505...
Wild plants of Vitis closely related to the cultivated grapevine (V. v. vinifera) are believed to have been first domesticated 10,000 years BC around the Caspian Sea. V. v. vinifera is hermaphrodite whereas V. v. sylvestris is a dioecious species. Male f
In brief, the hypocotyl was chopped into small discs with scalpel blade and immersed in 3–5 ml RNase-free enzyme solution (1.5% cellulase R10, 1.5% macerozyme R10, 0.4M mannitol, 10mM KCl, 10mM CaCl2, and 0.1% BSA). Then digested for 4 h with gentle shaking (30 r/min) in the ...
The growth medium contained the following (per liter): 0.1 g of KCl, 0.2 g of NH4Cl, 0.6 g of NaH2PO4, 10 ml of a vitamin solution [35], 10 ml of a trace element solution [35], 50 μl of a titanium citrate solution [36], 10 mM acetate and 40 mM fumarate. Cultivation was ...
coli as cellular factory are i) the loss of carbon in the form of acetate during aerobic growth at high growth rates, ii) the inhibition of cellular growth and protein production by acetate [2] and iii) the need for cofactor recycling, (namely redox coenzymes and free CoASH) to sustain ...
The Czapek Dox Broth (CDB) medium composed of 30 g sucrose, 3 g NaNO3, 1 g K2HPO4, 0.5 g MgSO4, 0.5 g KCl, 0.01 g FeSO4 and 500 mg chloramphenicol in 1 L water was used. The CDB medium was buffered with 100 mM of citric acid-sodium citrate buffer at pH 7.3. Only 10 mm ...