The term “Nanozyme” is a blend of “nanomaterial” and “enzyme, ” following a similar naming convention to that of ribozymes, abzymes, chemzymes, DNAzymes, synzymes, and others. Nanozymes, in comparison to natural enzymes and traditional artificial enzymes, offer benefits like affordability,...
The size of the pan-genome increased when a greater number of sequenced genomes are included, but the core genome decreased in size (Figure 4A). The predicted core genome and pan-genome of M. hyopneumoniae is shown in the form of a flower-plot schematic diagram (Figure 4B). Unexpectedly,...
After IEF, the proteins were equilibrated as described. First the IPG strips were incubated in 10 mL of equilibration buffer (6 M urea, 30% w/v glycerol, 2% w/v SDS, 50 mM Tris-HCl, pH 8.8) with 1% w/v DTT for 15 min, and then in the same solution containing 2.5% w/v ...
IEF of serum transferrin was performed using the method described by Carchon et al.[19]. Further delineation of the glycan structure was obtained by matrix assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) of serum transferrin. After PNGase treatment, the released N-glycans wer...
Samples were applied by overnight in-gel rehydration of 70 mm nonlinear pH 3-10 IPG gel strips. The first dimension (IEF) was performed at 6500 Vh for 3.5 h, using a Pharmacia LKB Multiphor II system. IPG strips were equilibrated with buffer in two steps. The first step employed 50 mM...
The advent of spectral CT has brought about a surge in radiomics research rooted in this technology. Dual-layer spectral detector CT (DLCT), a novel form of spectral CT capable of separating low- and high-energy photons, produces more precise images and furnishes more comprehensive energy-...
The first-dimension separation was performed using 18 cm Ready Strip IPG strips (non-linear, pI 3–10, GE Healthcare) for isoelectric focusing (IEF). The IPG strips were rehydrated with 400 μl rehydration buffer (7 M urea, 2 M thiourea, 2% (w/v) CHAPS, 65 mM DTT, 0.2% IPG buffe...
One milliliter of 2% lidocaine will be injected through the introducer needles to anesthetize the area before ablation. After placement of the introducer needle, a 18 gauge, internally cooled 4-mm active tip RFA electrode (Coolief, Halyard Health, Alpharetta, GA, USA) will be placed into the...
The dikaryotic stage is the form causing rust disease by attacking mesophyll tissues until pathogen injures the epidermis to release urediospores[5]. Barley resistance to leaf rust pathogens is governed by major resistance (R) genes (Rph genes) that are race-specific. Most Rph genes confer ...
Approximately 30 mg of protein were recovered at the end of the liquid-phase IEF pre-fractionation from an initial 60-mg load. The losses are accounted for by the multi-step pre-fractionation procedure, but are not the result of a precipitate that could not be dissolved in our lysis buffer...