HEK293-hTau cells were transiently transfected with 1 µg of plasmid expressing mCherry red fluorescence protein-GFP-microtubule-associated protein 1A/1B light chain 3 (mRFP-GFP-LC3) using 2 µl of Neofect™ DNA Transfection Reagent (TF20121201, Neofector, China) according to the manufact...
Additionally, this protocol allows the preservation and detection of expressed fluorescent proteins such as GFP alongside FISH probe signal. Finally, we establish an improved protocol for combined FISH and immunohistochemistry (IHC) that can detect RNA and protein in the same sample. This greatly ...
Generation of CLIC4-GFP knock-in mice A knock-in mouse that expresses GFP from the CLIC4 promoter was created to study the normally regulated expression of CLIC4 in mouse organs and tissues without concern for expression of other CLIC family members. The second exon was chosen for the fusion...
GFP and its derivatives also require an aerobic environment with sufficient cellular oxygen to form the fluorophore [5, 7–9]. Furthermore, detection of this fluorophore is completely inhibited in an oxygen-limited or anaerobic environment. The on-line measurement of GFP is feasible, but is not...
Upon grafting in human ovarian cortex, GFP and DDX-4 positive stem cells differentiate into GFP positive oocyte-like structure in vivo. Two populations of stem cells exist in adult ovaries including very small embryonic-like stem cells (VSELs) and ovarian stem cells (OSCs) in the ovary ...
Results of GFP intensity were represented as a ratio of GFP fluorescence to the culture density. All experiments were performed in at least three biological replicates. Significance of differences in GFP relative fluorescence between mutants and the wild type pBAD promoter was tested with t test....
Rhabdomeric photoreceptors of eyes in the terrestrial slug Limax are the typical invertebrate-type but unique in that three visual opsins (Gq-coupled rhodopsin, xenopsin, Opn5A) and one retinochrome, all belonging to different groups, are co-expressed. H
connected secondary neurons in different brain areas including the most distant ones (10 mm away from the injection site (IS)), 2) Tau species found in secondary connected neurons are mainly in a dephosphorylated form and 3) WT and mutated Tau species display differential spreading of Tau ...
Moreover, the inhibition of tumor growth by inducing p53/SIRT6/PARP1-dependent parthanatos was further verified in the xenograft mouse model. Results For the first time, we identified that inhibiting AKT triggered parthanatos, a new form of regulated cell death, leading to colon cancer growth ...
Western blot analysis of sucrose gradient-purified influenza viruses and a recombinant RBD protein using:Aanti-RBD rabbit polyclonal antibody (*) – influenza HA monomer with RBD insertion; (♦) – monomeric recombinant RBD (♦♦) – dimeric form of RBD; the higher bands are oligomers of ...