They were incubated together with the samples (sterile bacterial supernatant or microbially colonized ALIs’ basal media), negative controls (sterile bacterial growth media or basal media from vehicle treated ALI), or recombinant standards, and PE-conjugated detection antibodies, to form sandwich ...
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4a). Comparing the subpopulations, we found a considerable variation in terms of GCF values across various range categories (Fig. 4a). Similarly at gene level, there is variation of GCF values between subpopulations with PAR having the highest GCF burden (Fig. 4...
Besides, previous studies have also confirmed that microorganisms and ARGs in the environment can enter humans in the form of aerosols [34, 35]. Surprisingly, our results showed that the microorganisms and ARGs volatilized from the cat gut (feces) into the air were minimal, suggesting that ...
When the LTR subpart and internal are reported in separate consensus sequences, it is possible to use manual curation to find the ones that form one full-length LTR element (see main text). The number after “Final Multiple Alignment Size” indicates how many sequences were used in the ...
Subsequently, the filtered data were mapped to the Synechocystis reference genome in the NCBI Database (https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_000009725.1/). For gene expression analysis, raw sequence counts of known genes were quantified using the StringTie software. The expression ...
CPE and APN generate bioactive proteins through processing of pro-peptides, and both proteins can be found in either membrane-bound or soluble form [34, 35]. CPE is specifically expressed in enteroendocrine cells, a cell type known to be dysregulated in IBD [36]. Mice deficient of CPE have...
The influence of periodontal therapy on periodontitis, CKD and NETs concentrations was also evaluated. Results CKD patients had higher concentrations of NETs in plasma than non-CKD patients, and NETs concentrations were also increased in both GCF and plasma of patients with periodontitis than that of...
Nonetheless, the Indian buffalo reference genome assembly (NDDB_SH_1) (https://www.ncbi.nlm.nih.gov/assembly/GCF_019923935.1) provides better level of assembly and can be used for future research works on buffaloes. GATK software v.4.0.10.1 [90] was used for marking the duplicate reads, ...
Raw RNA-seq reads were first filtered using SOAPnuke (v1.5.6) [16] to obtain clean reads, which were then mapped to the reference genome (Ovis aries, GCF_000298735.2_Oar_v4.0) using HISAT2 (v2.1.0) and to the gene set using Bowtie 2 (v2.3.4.3) [17, 18]. Gene expression quanti...