first developed dominant-negative TGF-β receptor type II (dnTGF-βRII) transgenic mice on a C57BL/6 background by overexpressing a dominant-negative form of TGF-βRII with the CD4 promoter, which lacks a CD8 silencer [18]. Oertelt et al. first reported that these mice spontaneously ...
We are aware that alopecia due to barbering can manifest as a form of stereotypic behavior in C57BL/6 mice [24], but this was not observed in MyD88wt mice. At the age of 10 weeks, the weight of major internal organs was similar, but the weight of the spleen was increased in MyD...
This stabilized form of β-catenin can translocate to the nucleus, where it activates target gene transcription by complexing with T cell factor (TCF) and lymphoid enhancer-binding factor (LEF). In addition to key mediators of embryonic development, these target genes include critical growth-...
We previously isolated and cloned a fibroblast-like cell line from the kidneys of adult FVB/N mice [15]. These KMSCs were cultured on gelatin-coated dishes in minimum essential medium (MEM) with 10% horse serum (Gem Biotech, Woodland, CA, USA) as previously described [15]. For generation...
Three distinct reactive species, 3-methylglutaconyl CoA (MGc-CoA), hydroxymethyl-CoA (HMG-CoA), and methylglutaryl CoA (MG-CoA), react with lysine residues during leucine oxidation to form acyl-lysine modifications which serve as the substrates for SIRT-4-mediated deacetylation. Reactive acyl-CoA...
Immunohistochemistry with cleaved caspase 3 antibody revealed that oleuropein treatment led to higher levels of the cleaved form of caspase 3 in both epithelial and stromal cells of ectopic lesions than the vehicle treatment (Fig. 4b). Thus, oleuropein reactivated apoptosis in ectopic lesions by ...
Patients were informed about the details and purposes of the study and provided with written consent and in the case of their agreement and signing the consent form, they were included in the study. Therefore, informed consent was obtained from all subjects. Consent for publication Not applicable...
Besides their successful therapeutic application based on their intrinsic properties, MSCs also form an interesting cell source for the secretion of growth factors and cytokines, supporting CNS disease improvement [26]. Adopting this approach, the beneficial effect is induced by the secreted factors, wh...
The early events that occur following injection of single mammary basal stem cells into a cleared fat pad are obscure, however, cells that survive this process and can form outgrowths must, by definition, have the ability to survive being reduced to single cells and introduced to a new growth...
cell states interact with the tumor microenvironment to form organized systems capable of immune evasion, drug resistance, and metastasis by pan-cancer scRNA-seq and spatial transcriptomics analysis. The characteristics of the three transcriptome patterns described above are presented in Table1....