The column effluent was split after the column with three quarters going into the UV/electrospray ionization (ESI)-MS and one quarter into the ICP-MS using a short length of peek tubing. ESI-MS (Orbitrap Discovery, Thermo) was used for some samples in positive ES-FT-mode (resolution 30,...
For positive electrospray ionization source (ESI+) and negative electrospray ionization source (ESI-) analysis, samples were isolated using a 30 min linear gradient of solvent A (water spiked with 0.1% formic acid) and solvent B (acetonitrile spiked with 0.1% formic acid) as mobile phases. The...
The liquid chromatography-mass spectrometry system consisted of the Thermo Scientific UItimate 3000 ultra-high performance liquid chromatograph and the Thermo Scientific LTQ-Orbitrap XL mass spectrometer equipped with a electrospray ionization (ESI) source. The column was Waters Acquity PRM UPLC BEH C18 ...
The ankyrin repeats 1 and 2 form hydrophobic contact with the nuclear localization signal located at the dimer’s C-terminal domain, while ankyrin repeats interact with the RHD at the same terminus. The acidic property of the ankyrin repeats and PEST region repels the positively charged N-...
Non-targeted whole genome sequencing is a powerful tool to comprehensively identify constituents of microbial communities in a sample. There is no need to direct the analysis to any identification before sequencing which can decrease the introduction of
When individuals with the same HET genotype meet, they can produce a viable heterokaryon by anastomosis, whereas individuals with different HET genotypes form a fusion cell which is compartmentalized and undergoes a form of programmed cell death termed vegetative or heterokaryon incompatibility [35]. ...
Phytophthora root rot caused by the oomycete Phytophthora capsici is the most devastating disease in pepper production worldwide, and current management strategies have not been effective in preventing this disease. Therefore, the use of resistant variet
A family of double-bowl pseudometallocalix[6]arene discsElectronic supplementary information (ESI) available: Full experimental details on the synthesis of the ligands HL1and HL2available. CCDC reference numbers 758960–758962. For ESI and...
There is neither a complete component fingerprint of the SHL formula nor a comparative analysis on various SHL preparation forms. SHL is a mixture of three herbal extracts containing hundreds of compounds, and these compounds can further react with each other to form new compounds. In this work,...
Aβ peptides are classified into two major isoforms composed of 40 or 42 amino acids (Aβ40 and Aβ42), with Aβ42 being the more toxic form which plays an essential role in the pathogenesis of AD. We investigated whether YXT and its herbal components could inhibit the Aβ42 ...