Protein signals were visualised using the ECL kit (Beyotime, China) in a high-sensitivity chemiluminescent imaging system (Bio-Rad, USA). The grey scale values of each band were obtained using Image J, 1.54f (NIH Image J system, Bethesda, MD, USA). The relative expression levels of each ...
An Enhanced Chemiluminescence (ECL) Detection Kit (Millipore) was used to visualize the immunoreactive proteins. The information of antibodies: p53 (1:1000); p21 (1:1000). The antibodies against PYGL (Abcam, ab227403), p53 (Cell signaling, #2524S), p21 (Cell Signaling, #2947S) were used...
Colorectal cancer ranks as the third most prevalent form of cancer in the population and is well recognized as a significant global public health concern. Colorectal cancer has the potential to metastasize from the colon’s surface to the colon tissue and other organs such as the liver, prostate...
Liver metastasis is the leading cause of death in patients with colorectal cancer (CRC). Emerge evidence suggests that circular RNA (circRNA) is a pivotal player in cancer progression. However, its role in CRC liver metastasis remains largely unknown. Ci
Cells were maintained in Complete StemPro NSC SFM (A1050901, ThermoFisher, Waltham, MA, USA) to form neurospheres in 5–7 days. When the neurospheres were 150–200 μm in diameter, they were dissociated for subculture. For differentiation, the NSCs were harvested by centrifugation and plated...
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ECL Plex™ Fluorescent Rainbow™ Markers Quick View Millipore HAWP04700 MCE Membrane Filter, 0.45 μm Pore Size Quick View Millipore EZHAWG474 EZ-Pak® Membrane Filter Quick View Properties form liquid feature wetted part: no Full Range packaging pkg of 250 μL manufacturer/tradename Cytiva...
Cancer cells frequently evolve necroptotic resistance to overcome various survival stress during tumorigenesis. However, we have previously showed that necroptosis is widespread in head and neck squamous cell carcinoma (HNSCC) and contributes to tumor pr
3.Domestic demand:Various indicators such as automobile sales, passenger traffic, robust GST collections, rising electricity demand, and growth in household credit point towards sustained domestic demand. 4.Macroeconomic stability:Stable repo rates, government bond yields, exchange rates, and healthy forei...
The characterization of specific molecular signatures can be used to better understand the drug response in MDS. In this study, we found an elevated O-GlcNAc and OGT expression in CD34+ cells of MDS/AML patients who were non-responsive to DAC. O-GlcNAcylation, a ubiquitous form of PTMs, ...