The full-length mouse Engrailed-2 (En2) sequence was amplified from cerebellar mouse tissue via PCR and subsequently cloned into a pQE80L vector containing HIS/GST tag sequences. The EN2 protein was then produced in BL-21 bacteria and purified by passage over a GST affinity column. Following ...
Insecticide resistance (IR) is one of the major threats to malaria vector control programs in endemic countries. However, the mechanisms underlying IR are poorly understood. Thus, investigating gene expression patterns related to IR can offer important i
Abstract: A full-bridge diode-clamped multilevel LLC resonant converter suitable for power conver- sion systems that use high input voltage, such as railway vehicles, is proposed in this paper. In order to eliminate the voltage deviations of the capacitors connected in series to the high voltage...
A realizable k-ε model, which is better for rotational flow, is used for the simulation of turbulent flow. This model includes two additional equations in a tensor form: ρ ∂k ∂t + (V·∇)k =∂ ∂xj µ + µt σk ∂k ∂xj + Pk + Pb − ρε − YM + Sk...