1、打开Fullprof—单击ED.PCR,如图: 2、点击菜单栏中Templates—Simple Calculation—接着点击Cif,找到cif路径即可。 注意检查空间群,Fullprof偶尔会出现不识别空间群的情况,这时候手动输入就行,注意要空格,如下: Ok,之后点击保存。 3、或者点击快捷菜单:Cif...
2g). Similarly, full-length coverage with Smart-seq3xpress resulted in significantly (2–5-fold) increased read support over exon–exon and exon–intron splice junctions, which form the basis for RNA velocity inference16 (Fig. 2h). To perform a direct comparison of cluster granularity, we ...
It can also react with sulfide to form sulfur and thiosulfate. Furthermore, some bacteria can biologically convert tetrathionate to sulfur, sulfate, and thiosulfate (Sorokin 2003). Due to the above-mentioned conversions, tetrathionate is less likely to be available as an intermediate in the BD...
We developed a PCR-free cloning strategy allowing us to create repetitive DNA sequences encoding glutamine stretches of defined length. These sequences were used to generate improved constructs for filter retardation assays to study Huntingtin aggregation in vitro. Several methods have been described ...
3c). Taking the negatively charged wall of the microchannel as an example, when the liquid contacts the negatively charged wall of the microchannel, the cations in the liquid gather on the wall of the microchannel to form a thin electric double layer (EDL) (0.1 nm-10 nm). Under the ...
Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source o
500 ng of RNA pre-treated with DNase I (Fermentas) was used to prepare cDNA using the iScript cDNA synthesis Kit (Bio-Rad, USA, Hercules, CA, USA) according to the manufacturer’s directions. RNA was also extracted from OSE cells which were isolated as described above. RT-PCR was ...
The fundamental goal of transcriptomics is to generate and compare snapshots of mRNA populations. In order to be suitable for comparisons with new data sets, these transcription profiles have to consist of quantitative values stored in a digital form and allowing for normalization procedures compensating...
Q-RT-PCR was performed using an ABI 7500 real-time PCR machine (Applied Biosystems) based on Real Master Mix (YiSheng). β-Actin was used as the endogenous control gene. The 2−△△Ct method was used to obtain the relative expression levels of genes in the form of fold variation. ...
Two sources of error need to be considered in pyrosequenced amplicons. Those from the pyrosequencing itself and those introduced by the PCR amplification. The original implementation of PyroNoise simultaneously removed both sources of errors. Consequently it was necessary to align flowgrams. Here we ...