2g). Similarly, full-length coverage with Smart-seq3xpress resulted in significantly (2–5-fold) increased read support over exon–exon and exon–intron splice junctions, which form the basis for RNA velocity inference16 (Fig. 2h). To perform a direct comparison of cluster granularity, we ...
The cells in the spheres also retain the ability to differentiate in vitro to form cells representative of the three germline layers after five passages. Conclusions These data suggest that USSC growth mediumACF maintains USSCs in an undifferentiated state and supports growth in suspension. This is...
3). It indicates that Hal531 likely represents an ancestral form of Trp-halogenases that originated from non-actinomycetes such as alphaproteobacteria. However, this hypothesis requires further investigation. Since the function of these closely related halogenases in alphaproteobacteria is unknown, it ...
During the time between the 24 to 48 hour APF stages, the imaginal discs are still undergoing morphogenesis, but are close to their final adult form. The wings, leg muscles, abdominal bristles, abdominal muscles and internal genital ducts are all well formed, while further development of the ...
Guanine-rich sequences are able to form complex RNA structures termed RNA G-quadruplexes in vitro. Because of their high stability, RNA G-quadruplexes are proposed to exist in vivo and are suggested to be associated with important biological relevance. However, there is a lack of direct eviden...
of one zero-mode waveguide (ZMW). Next, fluorescently labelled deoxynucleoside triphosphates (dNTPs) are added into the wells and sequencing begins. The polymerase starts incorporating dNTP to the new DNA strand. Each incorporated fluorescent dNTP remains briefly at the bottom of the well, where a...
3' Hairpin OCPs design allows conversion of unused OCPs to inert double-stranded form. If the OCP is not circularized, the single stranded region of the unligated linear probe can bind P1 primer and be copied, a process that can lead to misamplification of unligated probe. To reduce the pot...
PCR was carried out using gene specific primers and expression was evaluated at the exponential phase of amplification. In brief, PCR was performed using 1 μl of cDNA template (diluted 10 times), 0.2 mM of dNTPs, 0.2 μM each of forward and reverse primers, 1 U of GoTaq® DNA ...
Artemis first, before ligation. Consistent with the critical role of DNA-PKcs in end-processing, DNA-PKcs null lymphocytes accumulate hairpin CEs, leading to T- and B- SCID in mouse models [49,50,51]. Meanwhile, DNA-PKcs null cells can ligate SEs to form signal joints (SJs) effectively ...
The HPV genome is packaged within the major capsid late proteinL1and the minor capsid proteins L2 [12,13]. FiveL1proteins form a pentamer, and 72 pentamers constitute the virus capsid. TheL1andL2proteins self-assemble into virus-like particles (VLPs) that induce high levels of neutralizing ...