2g). Similarly, full-length coverage with Smart-seq3xpress resulted in significantly (2–5-fold) increased read support over exon–exon and exon–intron splice junctions, which form the basis for RNA velocity inference16 (Fig. 2h). To perform a direct comparison of cluster granularity, we ...
Genetic kinship of ancient individuals can provide insights into their culture and social hierarchy, and is relevant for downstream genetic analyses. However, estimating relatedness from ancient DNA is difficult due to low-coverage, ascertainment bias, o
To test the noise removal algorithms we used eight previously published test data sets and one hitherto unpublished. These were all generated by preparing mixtures of known DNA sequences in known concentrations and amplifying and pyrosequencing. For the published data sets the standard GS FLX protocol...
Identifying pathogenic variants from the vast majority of nucleotide variation remains a challenge. We present a method named Multimodal Annotation Generated Pathogenic Impact Evaluator (MAGPIE) that predicts the pathogenicity of multi-type variants. MAG
an ecosystem, different species/populations interact with each other to form complicated networks through various types of interactions such as predation, competition and mutualism. on the basis of ecological interactions, ecological networks can be grouped as antagonistic, competitive and mutualistic ...
e Visualization of TLS-like domain detection of SpaDo and other methods (BayesSpace, SEDR, SpaGCN, Scanpy, and Seurat v4) on 4 RCC spatial transcriptomic datasets Full size image The spatial domains detected by SpaDo form a multi-resolution schema hierarchically, enabling the characterization of...
or water. cfDNA and exDNA are often used interchangeably to describe any form of DNA found in the interstitial space, circulation, or body fluids. This includes mitochondrial DNA (mtDNA), DNA associated with extracellular vesicles, foreign DNA, naked and free ambient DNA molecules, and DNA assoc...
In our study, we used high throughput sequencing to test the hypothesis that DNA polymerases have a bias for different oligonucleotides used as primers to initiate DNA synthesis. In order to define this source of DNA polymerase bias, we carefully examined the contribution of the DNA sequence ...
In recent years, stem cell therapy has become a very promising and advanced scientific research topic. The development of treatment methods has evoked great expectations. This paper is a review focused on the discovery of different stem cells and the pot
arithmetic mean, the outliers are presented in the form of circles/pluses; *p < 0.05, **p < 0.01, ***p < 0.001 (n = 6). Reprinted from [162] Full size image Another strategy to increase the transmigration of cells into brain tissue after intravascular infusion is to...