Contamination of heavy metals and antibiotics would threaten the water and soil resources. Phytoremediation can be potentially used to remediate metal and antibiotics contaminated sites. The current study was carried out over a period of 12 months t
lineages, which is one of the pathogenies of IH. The HemECs also result in a disorder of angiogenesis and further form hemangioma [7]. IHs possess kind of unique immunohistochemical markers, such as glucose transporter 1 (GLUT1), SALL4, and CD133 [7,8], which have important roles in th...
The activation of caspase-3 in DHA-treated ASTC-a-1 cells was examined by Western blotting which visualized the processing of the inactive caspase proform to the catalytically active, smaller units. For Western blot analysis, the cells were treated with 1 μM STS for 12 h, 20 μg/m1 DHA...
toruloides. As these two genes are expected to account for the majority of TAG formation [30], one possible explanation for the failure to obtain the double mutant is that the inability to form TAGs confers a severe fitness defect in R. toruloides....
1), proton NMR was sufficient for analyzing the number of methyl groups and their coupling pattern. The core structure of pamamycins and their biosynthesis have been described previously [37]. During biosynthesis, two asymmetric hydroxy acids are produced, which together form the final pamamycin ...
E. coli DH5α and BL21 (DE3) strains were purchased form TransGen Biotech Co., Ltd. (Beijing, China). The pMD™ 19-T Simple and pET-28a(+) vectors with His tags were obtained from TaKaRa Biotechology Co., Ltd. (Dalian China) and Novagen (America), respectively. Cloning of the ...
Epidermal growth factor receptor (EGFR) activating mutations play crucial roles in the tumorigenesis of human non-small cell lung cancer (NSCLC). The mechanism regarding how EGFR signaling regulates myeloid cell leukemia sequence 1 (Mcl-1) protein stabil
Using TILLING to identify novel genetic variation in each of the A and B genomes in tetraploid durum wheat and the A, B and D genomes in hexaploid bread wheat, we have identified mutations in the form of single nucleotide polymorphisms (SNPs) in starch branching enzyme IIa genes (SBEIIa)....
Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) hold great therapeutic potential in regenerative medicine. Therefore, it is crucial to establish a Good Manufacturing Practice (GMP)-compliant methodology for the isolation and culture of WJ-MSCs.
In fact, the main problems related to the use of E. coli as cellular factory are i) the loss of carbon in the form of acetate during aerobic growth at high growth rates, ii) the inhibition of cellular growth and protein production by acetate [2] and iii) the need for cofactor ...