After 24 h, the fluorescence intensity of the cells was measured. As expected, no fluorescence was observed in the negative control group indicating that Vybrant-DiO dye did not form nanostructures able to confound the interpretation of the results. Importantly, fluorescence intensity was similar ...
The proteins selected for fusions with CBD were highly reengineered, or ‘artificial’ (= not existing in Nature), which carried condensed functionalisation in the form of concatemers. These were composed of repetitive, bioactive epitopes, oriented in organised head-to-tail fashion, expressed ...
The integration of new Internet of Things (IoT) applications and services heavily relies on task offloading to external devices due to the constrained computing and battery resources of IoT devices. Up to now, Cloud Computing (CC) paradigm has been a good approach for tasks where latency is not...
while zeta potential was measured using laser Doppler electrophoresis (Otsuka ELSZ-2000 Series, Otsuka Electronics, Osaka, Japan). To ensure the physical stability of our formulations, we have conducted the time-elapsed measurements of DLS at the storage (4 °C) and physiological (...
In both use cases we monitored elders with biomarkers in the form of wearables (smartwatches and wristbands) while they performed an IADL, in this case, shopping. In particular, for the frailty use case, the elders wore a smartwatch, the Samsung Gear S3. In the second use case, dependence...
The power analysis revealed that we need a sample size of 50 independent computer runs, therefore all the DL algorithms have been run for 50 times in a complete tenfold cross-validation cycle. In Table 1 we present the performance results of the 5 DLs in terms on average accuracy (ACA) ...
LNP nanoparticles were diluted with ddH2O at a 1:100 ratio. Size measurements were taken at 25 °C with a 173° scattering angle using dynamic light scattering (DLS) on the Zetasizer Nano ZS90 (Malvern, UK). Zeta potential determinations, based on the electrophoretic mobility of the nanoparti...
Non-small cell lung cancer stands as the second most prevalent malignancy globally and is the foremost cause of cancer-related mortality, with an estimated 2 million new cases and 1.76 million deaths annually [1]. Owing to the absence of precise and effective early diagnostic methods, Owing to...
It has also been suggested that autolysin can weaken the peptidoglycan layer of bacteria, causing the inner membrane to protrude into the peripheral space and form OIMVs, which contain cytoplasmic contents [50]. Furthermore, recent studies have identified novel Gram-negative extracellular vesicles. ...
the vaccine was delivered in 81% of the cells from Toxo – donors, with an MFI of 11,698 units, and in 55% of the cells form Toxo + donors (p < 0.01), with an MFI of 7676 units (p < 0.05); for CD3−CD56+ cells, the vaccine was delivered in 79% of the Tox...