Complex II activity was measured by following the reduction of 80 μM 2,6- Dichlorophenolindophenol sodium salt hydrate (DCPIP; Sigma) at λ = 600 nm (ε = 19.1 mmol −1 cm –1) in the presence of 25 mM potassium phosphate buffer, 300 μM KCN, 1 mg ml−1 fatty acid...
A mutant form of 3-ketosteroid-delta(1)-dehydrogenase gives altered androst-1,4-diene-3, 17-dione/androst-4-ene-3,17-dione molar ratios in steroid biotransformations by Mycobacterium neoaurum ST-095. J Ind Microbiol Biotechnol. 2016;43:691–701. Article CAS PubMed Google Scholar Download ...
a biomass degrader of recalcitrant substrates. Their activity was assayed on cellulose in synergy with the cellobiose dehydrogenase from the same organism. We showed that the total release of oxidized oligosaccharides from cellulose was higher forPaLPMO9A,PaL...
Considering the fact that auxin inhibits axillary bud growth, while cytokinin relieves the growth inhibition [17,21], a greater number of spikelets in the cultivar having non-functionalOsCKX2compared with that having functionalOsCKX2[19] is plausible. In addition to promoting axillary buds growth,...
reaction lacking AD was monitored as a control. Activity is expressed as U/mg of protein after subtracting control data. One unit (U) of enzyme activity is defined as the amount of enzyme required for reduction of 1 µmol of DCPIP per minute (ε600 nm = 18.7 × 103L/mol·...
Poly(lactide-co-glycolide) acid nanoparticles (PLGA NPs) were synthesized using a double emulsion solvent evaporation technique [44,56]. First siRNA (DCAMKL-1 or scrambled) was condensed on the cationic polymer poly(ethyleneimine) (PEI, 5% w/v) to form an siRNA-PEI complex. siRNA-PEI (200...