4G). Given that the non-canonical eIF4G2 is required for YTHDF3-mediated translational regulation [22], we conducted Co-IP assay and confirmed that YTHDF3 and eIF4G2 form an endogenous complex in both SW620 and LoVo cells (Fig. 4H). As expected, silencing of eIF4G2 dramatically ...
Briefly, we removed the top 0–3 cm layer of soil, using the remaining soil as bulk soil. Each replicate consisted of a composite sample obtained by mixing three individual samples. The samples used for DNA extraction were stored at – 80 °C, while those for microbial cultivation form YR...
encoding VSVg, two packaging plasmids encoding rev, gag and pol genes, and a transfer DNA plasmid vector, was added to pre-warmed OptiMEM (Thermo Fisher Scientific, 31985070), followed by addition of PEIpro and incubation for 30 min at room temperature for the transfection complexes to form....
Microbiome alterations have been associated with depression, and fecal transfer of depressed patients’ microbiomes is sufficient to enhance despair behaviors in rodents. Yet little is known about the potential mechanisms, whereby microbes modulate depre
There have been substantial efforts to profile cancer dependency by loss-of-function screens in cell lines [1,2,3,4,5,6,7,8,9,10], providing valuable resources that can lay foundation for new ways to fight cancer. However, the screening methods are applicable only to in vitro cell cultur...
Classical Test Theory (CTT) is used to evaluate the quality of questions and assessment tests in learning environments using the statistical measures described in the following sections (Alagumalai and Curtis 2005;Ding and Beichner 2009;Doran 1980;Cohen et al. 2013;Erguven 2014). ...
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These expression cassettes were sequentially ligated to form a multi-gene expression cassette T7mXcP4HS–T7HsAADCS–T7HsAANATS–T7HIOMTS, and the EcoRI and HindIII digestion sites were designed at both ends of the sequence. The whole sequence was chemically synthesized and identified by Sangon ...
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Interestingly, Notch signaling plays a key role in fate selection of neuronal cells and we have previously shown that over expression of a constitutively active form of Notch-1 inhibits induced differentiation of neuroblastoma cells [23]. It is currently not clear whether a link between Pax-5 ...