This miR has been known to promote microglia polarization into the proinflammatory M1 form [77]. Although there are also contradicting studies that show miR-21-5p in a neuroprotective light [78, 79], it is generally agreed that miR-21-5p is involved in stress signaling and pathologic settings...
form local aggregation to ensure binary outcomes of the fate determination [19]. They further enrich other proteins for function, and produce precursors and differentiated daughter cells to meet the needs of microenvironment repair, while the other daughters retreat back into quiescence, thus preserving...
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A variety of cell death pathways play critical roles in the onset and progression of multiple diseases. Paraptosis, a unique form of programmed cell death, has gained significant attention in recent years. Unlike apoptosis and necrosis, pa
To investigate the colocalization of mitochondria with BNIP3 and Drp1, cells were incubated with a diluted Mito-Tracker Red CMXRos solution (#C1049B, Beyotime, Shanghai, China, 1:500) in the dark at 37 °C for 30 min. After fixation and permeabilization, the cells were then incubated wi...
Production of mtros ROS are natural byproducts of normal oxygen metabolism that are involved in cell differentiation, cell proliferation, and apoptosis, and ROS are maintained at normal levels in the form of superoxide radical anions and hydrogen peroxide. Homeostasis is associated with the pathologica...
Mitotracker-Red CMXRos was used to detect the morphology of the mitochondria. B At the indicated time points after UVA irradiation cells were harvested by sample loading buffer followed by immunoblotting. C Mitochondrial membrane potential was determined by JC-1 staining at 1 h-post UVA ...
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