Heindryckx F, Binet F, Ponticos M, Rombouts K, Lau J, Kreuger J, et al. Endoplasmic reticulum stress enhances fibrosis through IRE 1α-mediated degradation of miR‐150 and XBP ‐1 splicing. EMBO Mol Med. 2016. https://doi.org/10.15252/emmm.201505925. Article PubMed PubMed Central Go...
It has been demonstrated that serine could bind to palmitoyl-CoA to form sphingosine in ovaries, which regulates the proliferation and apoptosis of GCs through the PI3K/AKT/mTOR signaling pathway [21]. Our sequencing results showed that the activation of the PI3K/AKT signaling pathway was associate...
Then, cell imaging was made with an Olympus CKX41 Inverted Phase Contrast Microscope (Olympus, Tokio, Japan). For the estimation of binding kinetics by LigandTracer, Streptavidin-APC conjugate was used for ABR proteins detection. Flow cytometry Streptavidin-phycoerythrin (PE) was obtained from e...
coli. Benzyl alcohol was then condensed with acetyl-CoA by the alcohol acetyltransferase ATF1 from yeast to form benzyl acetate. In route II, a plant CoA-dependent β-oxidation pathway via benzoyl-CoA was assessed for benzyl alcohol and benzyl acetate production in E. coli. The overexpression ...
O2•− reduced nitro blue tetrazolium to form blue formazan, which was absorbed at 560 nm. SOD catalysed the dismutation of O2•− to produce H2O2 and O2, thereby inhibiting formazan formation. The deeper the blue colour of the reaction solution, the lower the activity of SOD. And ...
Both strains oxidise CO across a broad range of concentrations (< 100 ppmv – 10,000 ppmv), and genome sequence analysis revealed a candidate form-I carbon monoxide dehydrogenase (CODH), which is likely to catalyse this process. Each strain oxidised CO specifically at stationary phase but ...
Pulldown assays utilizing the p21-binding domain of PAK were employed to isolate the active form of Rac (GTP bound), followed by western blot analysis. The western blot images presented in panels A and E illustrate the levels of both total Rac and GTP-bound Rac in MDA-MB-231 and MDA-...
Verhoef S, Wierckx N, Westerhof RGM, de Winde JH, Ruijssenaars HJ. Bioproduction of p-hydroxystyrene from glucose by the solvent-tolerant bacterium Pseudomonas putida S12 in a two-phase water–decanol fermentation. Appl Environ Microbiol. 2009;75:931–6. CAS PubMed Google Scholar Pandey RP,...
The battle for biomass: a systematic review of food-feed-fuel competition. Glob Food Sec. 2020;25:100330. Article Google Scholar Blombach B, Grünberger A, Centler F, Wierckx N, Schmid J. Exploiting unconventional prokaryotic hosts for industrial biotechnology. Trends Biotechnol. 2021;40(4)...
In diabetic wounds, hyperglycemia-induced cytotoxicity and impaired immune microenvironment plasticity directly hinder the wound healing process. Regulation of the hyperglycemic microenvironment and remodeling of the immune microenvironment are crucial.