The development of the cortex is a delicate balance between proliferation, differentiation, and migration of neural progenitors (NPs). Throughout developmental process, various cellular mechanisms ensure that NPs differentiate into the correct cell subtypes, migrate to their correct regions, and form the...
In brief, the hypocotyl was chopped into small discs with scalpel blade and immersed in 3–5 ml RNase-free enzyme solution (1.5% cellulase R10, 1.5% macerozyme R10, 0.4M mannitol, 10mM KCl, 10mM CaCl2, and 0.1% BSA). Then digested for 4 h with gentle shaking (30 r/min) in the ...
Furthermore, studies have demonstrated that the combination of adjuvants and active components can form unique morphologies, which can alter the solubility, permeability, and intestinal absorption of the active components, ultimately enhancing their therapeutic effectiveness. Therefore, to comprehensively ...
Cells were cultured in Hank’s Balanced Salt Solution (HBSS, C0219, Solarbio) supplemented with CaCl2 and MgCl2. Subsequently, 10 µmol/L of DHE was added. The cells were incubated at 37 °C in a light-controlled environment for 30 min. The cells should be washed with HBSS and loaded...
[17] improved the original transformation system by adjusting the pH and CaCl2 concentration of the medium; the number of resistant plants increased by 2.7–6.4 times, the number of positive lines increased by 3–6 times transformation, and the genetic transformation efficiency increased by 5.7–...
The optimum temperature for activity was 35 ℃ for both free and immobilized cells but as the temperature was increased to 55 °C and above, the encapsulated form retained more activity than the free cells. The pH optimum shifted from 6.5 to 7 for the free cells to 7–7.5 for the ...
NK cells augment the anti-tumor activity of adaptive immune cells via cytokine secretion. However, metabolic interference in the form of lactic acid accumulation within the TME reduces intracellular pH in NK cells, inducing pH-dependent mitochondrial stress and metabolic disruption, ultimately promoting ...
The remarkable efficacy of these scaffolds is due to their unique structures, which infiltrate T cells to form dense cell-material clusters, thereby creating a microenvironment that enhances T cell activation and proliferation [97]. The clinical application of CAR-T cell therapy has been somewhat ...
conducted transcriptome sequencing analysis on jujube seedlings to study the molecular mechanism by which CaCl2 alleviates salt stress in jujube [19]. Wen Zishao et al. used the Illumina HiSeq 4000 platform to sequence the transcriptome of jujube kernels at different developmental stages [20]. Li ...
Secondary malignant bone tumors originate from other organs or tissues and metastasize to the bone. The most prevalent form of secondary malignant bone tumor is bone metastasis (BM) [3]. Apart from metastatic bone tumors, secondary malignant bone tumors also include leukemic infiltration of bone, ...