Status epilepticus (SE) is a common severe neurological emergency. Cerebral edema caused by SE is unavoidable and may exacerbate epilepsy. Recent studies have identified cerebrospinal fluid (CSF) as a crucial fluid source of initial cerebral edema follow
Data analysis was conducted using analysis of variance (ANOVA) followed by the Tukey post hoc test for parametric data, or the Kruskal–Wallis test followed by Dunn’s test for nonparametric data. The data are presented in the form of the mean ± standard error of the mean (SEM). In...
Transplantation of human umbilical cord-derived mesenchymal stem cells into 2-OA-bovine serum albumin (BSA) mice considerably ameliorates hepatic inflammation by dampening the Th1/Th17 response [62]. Moreover, the absence of Th17 cells inhibits hepatic accumulation of IFN-γ-producing cells, but IL...
For ethical reasons mice were sacrificed when tumors reach a maximum volume of 1,500–2,000 mm3. Immunohistochemistry Formalin-fixed, paraffin-embedded (FFPE) mouse tumor tissue sections (10 μm) were incubated with 3% H2O2 for 5 min and then blocked with 10% goat serum and 1% BSA in PBS...
Mesenchymal stem cell (MSC) transplantation therapy is highly investigated for the regenerative repair of cartilage defects. Low-intensity pulsed ultrasound (LIPUS) has the potential to promote chondrogenic differentiation of MSCs. However, its underlyin
Equal amounts of proteins (30 µg) were subjected to SDS-PAGE and transferred to PVDF membranes. The membranes were blocked with 5% BSA for 2 h and incubated with primary antibodies overnight at 4 °C. Primary antibodies including anti-CitH3 (1:1000, ab5103, Abcam, Cambridge, England,...
Metabolism is increasingly recognized as a key regulator of the function and phenotype of the primary cellular constituents of the atherosclerotic vascular wall, including endothelial cells, smooth muscle cells, and inflammatory cells. However, a compreh
Next, antigen retrieval was performed by heating the sections in sodium citrate buffer at a high temperature for 10 min. Endogenous peroxidase activity was blocked by treating the sections with 3% H2O2. Then, nonspecific binding was blocked with 3% BSA (BIOFROXX, 4240GR100) for 1 h. The ...
Current challenges in nursing care With a global nursing workforce of 27.9 million, nurses represent the largest occupational group in the health sector and form the backbone of the health care system [1]. However, the shortage of nurses is a major concern in most of the world’s countries,...
Amplicon-based mycobiome analysis has the potential to identify all fungal species within a sample and hence could provide a valuable diagnostic assay for use in clinical mycology settings. In the last decade, the mycobiome has been increasingly characte