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Following a 96-hour incubation period at 37 °C, the migration of cells towards the wound site was captured using light microscopy, and the migration area was quantified using Image J software. (2) For tumor invasion assay, MCF-7 cells were cultured to form spheres in hanging drops of ...
In natural medicinal chemistry research, the active ingredients found in TCMs primarily exert their efficacy through a population of small molecules, which includes oligosaccharides, saponins, terpenoids, and alkaloids, among others [43]. However, the in vivo delivery of small molecule drugs is largely...
in the first phase of infection, pathogens secrete plant cell wall-degrading enzymes (CWDE): polygalacturonases (PG), pectin lyases (PL) and pectin methylesterases (PME), as well as cellulases and hemicellulases [2]. In turn, the degradation of the plant ...
Efforts should be made to accelerate the transformation of the traditional manufacturing industry in terms of digital, internet and intelligent technologies, and extend the industrial chain upstream and downstream, so as to form a complete industrial chain and industrial cluster. ...
When deposited on APTMS-treated mica in the presence of 100 mM NaCl, highly −SC plasmids (σ = −0.04) assumed a plectonemic form with several crossings seen in each molecule and, occasionally, irregularly balled-up or aggregated forms (Fig. 2A). These shapes were already reporte...
Skin cancer is a prevalent form of cancer worldwide, and gaining insight into its development is essential for the development of prevention and treatment strategies. The three major types of skin cancer are basal cell carcinoma, squamous cell carcinoma, and melanoma, while merkel cell carcinoma, ...
discussions form the foundation for the proposed definition of the term microbiome, and the set of amendments containing the rules and baselines for microbiome research is described here. Moreover, this article provides an overview on the historical development of microbial research and shows how this...
of computational methods for some form of single-cell data, and not a primary method publication; see Additional file1for the list of studies). We then designed a questionnaire to query various attributes of a benchmark study (seeMethods, Additional file2) and crowdsourced the review of these...
Besides, we also utilize the binary classification metrics to evaluate the performance for each therapeutic peptide function prediction task in a one-versus-all form, including the area under the ROC curve (AUC) [17], Matthews’s correlation coefficient (MCC) [18], the F1 measure [19], and...