It has been suggested that learning is especially successful, if it occurs in an environment close to reality and enhances an active construction of knowledge, rather than passively absorbing it in the form of lectures, for example [40]. This so-called situated learning is an essential basis ...
Most of the therapeutic proteins approved so far have been obtained using transgenic hamster cell lines, namely 49 in chinese hamster ovary cells (CHO) and 1 in baby hamster kidney cells (BHK) (Additional file 1). The main advantage of this expression system is that cells can be adapted to...
The human immunodeficiency virus type 1 (HIV-1) has been intensely investigated since its discovery in 1983 as the cause of acquired immune deficiency syndrome (AIDS). With relatively few proteins made by the virus, it is able to accomplish many tasks, w
Commonly used media for the differentiation of human pluripotent stem cells into cardiomyocytes (hPSC-CMs) contain high concentrations of proteins, in particular albumin, which is prone to quality variations and presents a substantial cost factor, hampering the clinical translation of in vitro-generated...
The ATP-binding cassette A1 (ABCA1), a transmembrane protein, plays a crucial role in transporting intracellular free cholesterol to lipidate ApoE and form HDL-like particles for cholesterol transport in the brain [25–28]. Recycling of ABCA1 to the plasma membrane is critical for cholesterol ...
However, a 1000-fold reduction was found in virus titer 24 h post-infection of BHK cells expressing small hairpin RNA (shRNA) under the control of a mouse U6 promoter from a plasmid construct [35]. We observed a significant cross-inhibition of FMDV replication in BHK-21 cells by siRNAs ...
The clinical presentation of rabies encephalitis in humans is in two distinct forms- the classical form which represents ~80% of all cases, also known as the “encephalitic or hydrophobic rabies” and the non-classical form which is also known as “paralytic” or “numb” rabies. The ...
we compared the biochemical properties of rFVII synthesized in human embryonic kidney-derived (HEK)293 cells (HEK293rFVII) with those of rFVII expressed in Chinese hamster ovary (CHO, CHOrFVII) and baby hamster kidney (BHK, BHKrFVII) cells, and also with those of plasma derived FVII (pdF...
Helical nucleocapsids comprise the negative-sense, single-stranded RNA genome tightly associated with the 52 kDa nucleoprotein (N) which, together with the 43 kDa phosphoprotein (P) and the large multi-functional enzyme (L) form a ribonucleoprotein complex [10]. Nucleocapsids are encased in the ...
Maintenance of BHK-21 cells was performed using standard procedures. The conjugate stoichiometries were approximately Dx(70):Val = 1:50; WGA:Dx(70):Val = 2:1:50. In terms of valacyclovir (w/w), the free Val:Dx-Val:WGA-Dx-Val ratio is assumed to be 1:0.1:0.08 (i.e. correcting...