Patients meeting the inclusion criteria and undergoing routine neurological examinations at the clinic during the study period were included in this study upon completing the ethical consent form. The characteristics of the qualified patients were collected through interviews such as age, gender, employment...
Brazilian Food Insecurity Scale BFP: Bolsa Família Program PHU: Primary Health Unit CPB: Continuing Payment Benefit ES: Elementary school FAO: Food and Agriculture Organization FI: Food insecurity HS: High school MW: Minimum wage PR: Prevalence ratio UE: University education ...
Transduced cells were enriched based on the blue fluorescent protein (BFP) signal by fluorescence-activated cell sorting (FACS) using FACSAria SORP (BD Biosciences). We named this cell line CRISPRi-U87. CRISPRi screening The workflow of the CRISPRi screen is illustrated in Fig. 3D. The H1 ...
For induction of DSB formation, the I-SceI-expressing plasmid and HR donor (pCAGGS DRR mCherry Donor EF1a BFP) plasmid were cotransfected into the above selected cells. After 48 h, transfected cells were harvested and examined for the expression of GFP and mCherry by CytExpert flow cytometry ...
FP GFP BFP SFP HFP CFP <Round>BA (m6)BPA (p6)BG (g6) <Diamond>BD (m6)BPD (p6)DG (g6) 1 +0.008 +0.002 +0.012 +0.006 -0.002 -0.008 1.20 (1.50)~2.50 2, 3 1.0~5.0 0.1 0.5 0 0.6 2 2.50~6.00 (4.00) 2~6 1.0~15.0(10.0) 0.5 1 1.2 3 3.50~6....
Brain tumours are the leading cause of cancer-related death in children, and there is no effective treatment. A growing body of evidence points to deregulated epigenetics as a tumour driver, particularly in paediatric cancers as they have relatively few
Since both the Cre/loxP and the Flp/FRT systems leave behind a footprint in the form of a single loxP or FRT site after excision, an alternative method is the piggyBac transposon system which mediates “scarless” excision by removing transgenes flanked by piggyBac-specific inverted terminal ...
ADAM10 is activated by proteolytic cleavage of its prodomain via convertase proteins. To express a catalytically active ADAM10 protein, a variant lacking the prodomain (ACT) was generated. In addition, a truncated form also devoid of the metalloproteinase domain (ΔMP) was cloned, which allowed ...
Vlenterie R, van Gelder MMHJ, Anderson HR, Andersson L, Broekman BFP, Dubnov-Raz G, et al. Associations between maternal depression, antidepressant use during pregnancy, and adverse pregnancy outcomes: an individual Participant Data Meta-analysis. Obstet Gynecol. 2021;138(4):633–46. Article...
This, in turn, led to activation of ERK1/2, which stabilized the MYC protein. Furthermore, KLF16 interacted with MYC to form nuclear condensates, thereby enhancing MYC’s transcriptional activity. Additionally, MYC transcriptionally upregulated KLF16, creating a positive feedback loop between KLF...