Nutlin, a selective MDM2 inhibitor, is a negative regulator of the tumour-suppressor gene TP53, triggering apoptosis and cell cycle arrest exclusively in cancer cells retaining the wild-type TP53 form [70]. As depicted in Fig. 2C, seven of the 24 cell lines within this dataset harbour a ...
CRC development may take decades to transform into the final form of cancer. Many factors could modify the pace of this process, including diet, alcohol, and tobacco consumption, as well as contaminants present in water and food [5, 6]. Recently, the gut microbiota (GM) has also been ...
RNA profiling technologies at single-cell resolutions, including single-cell and single-nuclei RNA sequencing (scRNA-seq and snRNA-seq, scnRNA-seq for short), can help characterize the composition of tissues and reveal cells that influence key functions in both healthy and disease tissues. However...
Moreover, distinct populations of resident immune cells in the corneal EP, including innate lymphoid cells, Langerhans cells, mast cells, macrophages, and T cells, form a complex immune network that enables the Cor to mount prompt immune responses to different environmental challenges [17, 18]. ...
2g). Similarly, full-length coverage with Smart-seq3xpress resulted in significantly (2–5-fold) increased read support over exon–exon and exon–intron splice junctions, which form the basis for RNA velocity inference16 (Fig. 2h). To perform a direct comparison of cluster granularity, we ...
The following cell lines were used in this study: human embryonic kidney cell line 293T (ATCC, Manassas, USA), mouse B cell lymphoma line A20 (ATCC), mouse metastatic lung cancer cell line 344SQ (University of Texas M.D. Anderson Cancer Center, provided by Jonathan M. Kurie), human pan...
Next, to validate whether the downregulated YAP1 rewires stromal cells for enhanced production of stimulatory growth factors such as GROα to form a premetastatic niche in vivo, stromal-specific Yap1 conditional knockout (cKO) mice bearing two loxP sites flanking the exons of the Yap1 gene were...
Ferroptosis, a non-apoptotic form of cell death induced by accumulation of free iron ions and lipid peroxidation, its importance for cancer treatment is gradually being recognized. Research on the anti-cancer mechanism of juglone is accumulating. However, the specific mechanism by which it directs ...
Each program outputs some form of variant call file (VCF), from which we extracted SNV calls along with associated information provided by the variant caller, such as depth of coverage, alternative allele frequency, p-value, etc. For ITVC, the results on the Oncomine Focus data were obtained...
There, they are subject to reactions orchestrated by the periplasmic enzymes ScyD, ScyE, ScyF, DsbA, and TyrP to produce the reduced form of scytonemin. Once secreted to the extracellular matrix, it auto-oxidizes and takes on its final yellow-brown appearance. Parallel studies suggest that a...