2g). Similarly, full-length coverage with Smart-seq3xpress resulted in significantly (2–5-fold) increased read support over exon–exon and exon–intron splice junctions, which form the basis for RNA velocity inference16 (Fig. 2h). To perform a direct comparison of cluster granularity, we ...
Metabolic engineering has been enabling development of high performance microbial strains for the efficient production of natural and non-natural compounds from renewable non-food biomass. Even though microbial production of various chemicals has successfully been conducted and commercialized, there are still...
2. Prediction of operons Multi-gene operons were predicted using our JPOP program [27, 28] for each genome. For genes arranged in tandem on the same strand and not predicted to be part of an operon by JPOP, we considered them to form an operon if their intergenic distances are shorter...
It is then converted into the compatible solutes ectoine and 5-hydroxyectoine, respectively, by the L-2,4-diaminobutyrate transaminase (EctB; EC: 2.6.1.76) to form L-2,4-diaminobutyrate, a metabolite that is then acetylated by the 2,4-diaminobutyrate acetyltransferase (EctA; EC: 2.3.1.178...
3). It indicates that Hal531 likely represents an ancestral form of Trp-halogenases that originated from non-actinomycetes such as alphaproteobacteria. However, this hypothesis requires further investigation. Since the function of these closely related halogenases in alphaproteobacteria is unknown, it ...
anti-GBM activity; however, infusion toxicities and difficulty in penetration through the blood−brain barrier (BBB) are current challenges that limit the systemic treatment of GBM with an IgG1 form of anti-CD47 antibody. In this work, we generate an oHSV carrying a transgene encoding a ...
Each program outputs some form of variant call file (VCF), from which we extracted SNV calls along with associated information provided by the variant caller, such as depth of coverage, alternative allele frequency, p-value, etc. For ITVC, the results on the Oncomine Focus data were obtained...
To select antibodies against native form of CD9 on cell surface, cell panning was performed using a stable CD9 transfectant, HEK293-CD9. The library phages were pre-cleared by subtraction panning with parental HEK293 to deplete non-specific binding phages. The pre-cleared phages were incubated...
cereus ATCC 14579 stand out as having a much higher fraction of SNPs, indicating a larger phylogenetic distance from both pooled ISS and pooled SAF samples in this study. No B. cereus bases were covered at depth from SAF reads; therefore, no fixed substitutions were detected. B. cereus ...
Next, to validate whether the downregulated YAP1 rewires stromal cells for enhanced production of stimulatory growth factors such as GROα to form a premetastatic niche in vivo, stromal-specific Yap1 conditional knockout (cKO) mice bearing two loxP sites flanking the exons of the Yap1 gene were...