The invasion of phytopathogens impacts the composition and associations of the internal microbial inhabitants. Leaf spot is one of the most devastating diseases in eggplant var. Mattu Gulla which is unique in terms of geographic indication (GI)
Many problems in computational biology require alignment-free sequence comparisons. One of the common tasks involving sequence comparison is sequence clustering. Here we apply methods of alignment-free comparison (in particular, comparison using sequence
The conference aims to popularize bioinformatics and promotes active application of bioinformatics in agricultural and biomedical fields of research; identify new trends in the fields of bioinformatics, computational genomics and transcriptomics, as well as in sequencing of biologically active molecules and t...
A likely explanation would be a differential mutational pattern in cancer driver genes that underlie such a differential proliferative pathway. Chronic insulin, glargine, X10, or IGF1 treatment did not result in more mutations, and no correlation could be detected with the number of mutations or ...
(as assessed on the capillary sequencer) and the selective nucleotides added to the primers used to generate the TDF, GenFrag can identify the corresponding sequence of a TDF and thereby the identity of the gene corresponding to the TDF. The GenFrag software is developed as open source ...
The Asian tiger mosquito, Aedes albopictus, is one of the 100 worst invasive species in the world and the vector for several arboviruses including dengue, Zika and chikungunya viruses. Understanding the population spatial genetic structure, migration, an
“pollen-tube mimicry” but must have other reasons. One putative explanation might be a partially mutualistic mode of theC. purpurea-rye interaction which has been discussed earlier [39,40].C. purpureacauses only limited damage to the host, as only single ovaries of rye plants are infected ...
(primers indicated in Additional file1) and size fractionated on an Applied Biosystems 3730 capillary sequencer (Applied Biosystems, Foster City, CA, USA). Then, 1 μl of the PCR product was diluted 1/70 and run on an agarose gel to confirm amplification, and 1 μl of the diluted PCR ...
(Illumina Inc., San Diego, CA, USA) according to the manufacture’s instructions. The libraries were pooled, multiplexed and run across eight lanes of four 1x50 flow-cells, using Illumina TruSeq chemistry (version 3.0) and a HiSeq2000 sequencer (Illumina Inc., San Diego, CA, USA). Due...
according to the procedure described [70]. Input and IP samples were amplified using a SEQXE WGA Kit (Sigma-Aldrich, Dorset, UK) before a clean-up step using a QIAquick Cleanup Kit (Qiagen, Manchester, UK). Samples were then sequenced on the Ion Torrent semiconductor sequencer using the ...