A simplified example of the ABEA algorithm and a representative dynamic programming table is shown in Fig. 2. Algorithm 1 summarises the ABEA algorithm and the reader may refer to Supplementary Materials for a detailed explanation. Fig. 2 Nanopore raw signal, events and ABEA algorithm. In ...
Mitochondrial proteins (MP) are the proteins localized in mitochondria and are key component to mitochondrial functions [8]. There are an estimated 1500 proteins in mitochondria of rats [9], participating as components of electron transport chain, metabolic pathways, and factors for replication, initi...
In paired-end sequencing, the sequencer produces two reads for each DNA fragment. Suppose that the fragment length is m (bases), and the lengths of forward and reverse reads are both n. An overlap between the two reads occurs when m < 2n. The sequence of a fragment is denoted by S =...
while the proportion ofAe. albopictustoAe. aegyptiwas approximately 1:2 in 2014 in Jinghong city, Yunnan Province [18].Aedes albopictushas been reported to be the sole mosquito vector for dengue transmission in Guangzhou, with no presence ofAe. aegypti...
(Illumina Inc., San Diego, CA, USA) according to the manufacture’s instructions. The libraries were pooled, multiplexed and run across eight lanes of four 1x50 flow-cells, using Illumina TruSeq chemistry (version 3.0) and a HiSeq2000 sequencer (Illumina Inc., San Diego, CA, USA). Due...
A likely explanation would be a differential mutational pattern in cancer driver genes that underlie such a differential proliferative pathway. Chronic insulin, glargine, X10, or IGF1 treatment did not result in more mutations, and no correlation could be detected with the number of mutations or ...
(primers indicated in Additional file1) and size fractionated on an Applied Biosystems 3730 capillary sequencer (Applied Biosystems, Foster City, CA, USA). Then, 1 μl of the PCR product was diluted 1/70 and run on an agarose gel to confirm amplification, and 1 μl of the diluted PCR ...
Early life exposure to adverse environments affects cardiovascular and metabolic systems in the offspring. These programmed effects are transmissible to a second generation through both male and female lines, suggesting germline transmission. We have pre
(NEB Q5, New England Biolabs), and deep sequencing was performed at the OMRF Clinical Genomics Center on an Illumina miSeq sequencer using a 300 bp paired-end sequencing protocol, as previously described [20]. Microbial analyses were performed using the Quantitative Insights into Microbial Ecology...
Reviewer comments:R2_5, “Page 3, Lines 5-8: I think that it would be good to give a better explanation of the hypothesis underlying the MetaBinG2 method. Specifically, how does the method know a priori, which are the most abundant organisms in the samples when assigning weights?” ...