Plasma concentrations of LDH and CK-MB were measured using an automatic biochemical analyzer (Hitachi, 7600), whereas cTnI was measured by a Quantikine mouse ELISA kit (Cusabio, CSB-E08421). Measurement of blood pressure Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were ...
Blood phosphorylated tau (p-tau) forms are promising Alzheimer’s disease (AD) biomarkers, but validation in matrices other than ethylenediaminetetraacetic acid (EDTA) plasma is limited. Firstly, we assessed the diagnostic potential of p-tau231 and p-tau181 in paired plasma and serum samples. Se...
Fig. 1 The cAMP signaling pathways. The extracellular cAMP can be converted into AMP by plasma membrane-anchored ecto-PDE, followed by the conversion of AMP into adenosine by CD73. Ligands-engaged Gs- or Gi-coupled receptors may stimulate or inhibit cAMP synthesis, respectively. The ABCC transp...
Furthermore, we investigated if observed diffential gene expression is related to sex steroid hormone levels in placenta and umbilical cord blood plasma, and may have a programming effect on offspring body composition. To answer these questions, we analyzed the gene expression in the placentas of ...
Blood processing Non-fasting blood was collected via venipuncture from study participants into both serum-separating tubes (SST) and ethylenediaminetetraacetic acid (EDTA)-containing tubes, followed by centrifugation at 2000 g for 10 min at 4 °C. Liquid chromatography–tandem mass spectrometry (LC–...
VL measurement is mostly done with laboratory assays, and fresh plasma is the preferred sample type. However, the cold chain system for blood sample transportation and 24-hour time window requirements preclude the use of this sample type in rural and remote areas. Dried blood spot (DBS) is ...
Red blood cells (RBCs) are the most abundant cells in the body, possessing unique biological and physical properties. RBCs have demonstrated outstanding potential as delivery vehicles due to their low immunogenicity, long-circulating cycle, and immune characteristics, exhibiting delivery abilities. There...
Also, an evaluation of the biomarkers in other sample types, such as whole blood and red blood cell lysates should be performed, since in a previous study these samples provided additional interesting information about the redox status in dogs [54]. In addition, further studies should be made ...
New-onset diabetes was defined as a FPG value ≥ 126 mg/dL and/or a plasma glucose concentration ≥ 200 mg/dL after a 2-h 75-g oral glucose tolerance test. Biochemical analysis During the baseline visit, peripheral venous blood samples were obtained in the morning after an over...
The difference in AST between pre- and post-treatment blood samples was not considered to be statistically significant. One animal exhibited elevated GGT following treatment, but overall change in GGT for all animals was not statistically significant. Statistically significant increases were seen in ...