inhibitorsalvage pathwayFucosylation of glycans impacts a myriad of physiological and pathological processes. Inhibition of fucose expression emerges as a potential therapeutic avenue for example in cancer, inflammation, and infection. In this study, we found that protected 2‐fluorofucose 1﹑hosphate ...
(9) and known fucosylation inhibitor P-Fuc2F. To further evaluate the potency of Fucotrim I (9) and II (10), we made a direct comparison with known fucosylation inhibitors P-Fuc2F, P-l-Fuc6F3(Fucostatin I) and P-l-Fuc-1CH2P (Fucostatin II) (Fig.3c)5,6,7. The EC50values ...
PKA and actin signaling have been reported to mediate T cell activation, motility and immune synapse formation15,16 suggests that l-fuc promotes T cell trafficking to the tumor, a notion confirmed using an SW1 melanoma C3H mouse model treated with or without FTY720 (an inhibitor of lymph node...
As a proof-of-concept experiment to test whether FUT8 could be a potential metastatic target, we used pharmacological inhibition with a general fucosylation inhibitor, 2-fluorinated-peracetyl-fucose [20]. MDA-MB-231 cells were treated with this FUT inhibitor for 5 days, and inhibition of core ...
After washing three times with 250 μl of washing buffer, 30 μl of the reverse transcription mix (5 mm MgCl2, 1× reverse transcription buffer, 1 mm dNTP, 0.4 U of recombinant RNasin RNase inhibitor, 0.4 U of reverse transcriptase, 0.5 μg of random hexamers in nuclease-free water) ...
Phosphodiesterase inhibitor ameliorates senescent changes of renal interstitial pericytes in aging kidney. Aging Cell. 2024;23:e14075. Article CAS PubMed Google Scholar Kuppe C, Ibrahim MM, Kranz J, Zhang X, Ziegler S, Perales-Patón J, et al. Decoding myofibroblast origins in human kidney ...
Overall, the results presented here suggest that 2F-Fuc is a metabolically incorporated inhibitor of plant cellular fucosylation events, and potentially suggest that other 2-fluorinated monosaccharides could serve as useful chemical probes for the inhibition of cell wall polysaccharide biosynthesis. 展开 ...
HEK293 cells (1 × 107 cells) were homogenized in 2 ml of lysis buffer (50 mm Tris-HCl, pH 7.4, 0.1 m NaCl, 1 mm EDTA, and protease inhibitor mixture (Roche Applied Science)) using a Polytron homogenizer (seven times for 15 s in an ice bath), followed by centrifugation at 2,000...
XU, PINGKHETAN, ANURAGPARSONS, RODNEY LAWRENCESMITH, MICHAEL J.SCHMIDT, MICHAEL ANTHONY
Treatment with 2-fluorofucose (2-FF), a fucosylation inhibitor, completely abrogated its sLeX/A expression and dramatically reduced adherence of CF1_T cells to E-selectin under hemodynamic flow conditions. In addition, 2-FF treated CF1_T cells showed a reduced migratory ability, as well as ...