Number five. It was literally impossible for me to poop while sitting in a bed. The hospital didn’t have a hoyer lift. They had rooms that had lifts built into the ceiling, and for some brilliant reason they hadn’t put me in one of those. So I could not be transferred to a comm...
Total RNA was extracted from cells collected from the knock-down and complementation experiments using the Bacteria RNA Mini Kit with DNase (Geneaid Biotech Ltd., New Taipei, Taiwan). The complementary DNA (cDNA) was prepared from the total RNA by reverse transcription using the Invitrogen™ Sup...