A total of nine spectra were obtained for each pupal development age (days) of S. peregrina at each constant temperature (20 °C, 25 °C, and 30 °C) and averaged to a single spectrum to reduce systematic errors. The sample spectra were automatically removed from the background spectra ...
Five replicate spectra of each sample (50 scans/spectrum using nominal resolution of 4 cm−1) were recorded in the mid-infrared region (4000–400 cm−1) using five different aliquots of the sample. An average spectrum was determined for each sample (partial inhomogeneity of raw propolis ...