FTIR技术就是利用上述原理,通过分析组织细胞各相应基团的分子振动变化来检测细胞的物质组成、结构域构象变化等生化指纹.在实际操作中,测量红外光谱中各谱带的峰位(P,peak position)、峰高(H,peak height)、峰强(I,peakintensity)、峰面积(A,peak area)以及半高宽(FWHM,fullwidth at half um)等指标并计算不同谱...
at different postmortem time points.All spleen tissues were measured using FTIR spectrometer.ResultsThe absorption peak position showed no changes after death,but absorption intensity displayed obvious changes: (1)the absorption intensity assigned to nucleic acid (1080 cm-1and 1238 cm-1) showed ...
图4 甘油和甲醇的红外特征吸收峰Fig.4 Absorption peak of IR spectrum of glycerol and methanol 甘油初始浓度较大,采用培养开始时无甘油的培养基作为背景,选取甘油红外谱图中1 140～950 cm-1的波数范围,在0.5～50 g/L配置了28个不同浓度水平的甘油-培养基溶液,其中17个作为建模集,11个作为验证集;由于甲醇初...
红外光谱FTIR课件.ppt,吡嗪酰胺(抗结核病药) 丙二烯类 两个双键共用中间碳原子,耦合强烈,1600厘米-1无吸收,在2000-1915厘米-1和1100-1000厘米-1附近有不对称和对称伸缩振动,两峰相距900厘米-1,前者为中强峰,后者为弱峰。 芳香烃 振动类型 波数(cm-1) 说明 芳环C-H
of the ATR crystal area. This keeps the liquid from vaporizing when the sample chamber is depressurized and enables simple ATR measurements. The Si prism in the ATR accessory can provide a penetration depth of approximately 2 μm at 1000 cm -1 and approximately 10 μm at 200 cm -1....
The shifting characteristics of the absorption peaks in the range 800-1025 cm(-1) for samples at the ages of 8-12 h were comprehensively investigated in this study. The research results showed that the shifting speed of the characteristic peak for the blank sample was exactly the same as ...
Infrared spectrum chart peak positions were basically the same, and the chemical composition were roughly the same. They were composed of carbohydrate, protein, lipid and other biological molecular vibration spectrum band.From 1 800 to 1 000 cm-1 for second derivative conduction, the difference ...
5. The peak at 976 cm−1 shows the presence of CrO3 and other peaks at 826, 890 and 942 cm−1 show the presence of Cr(VI) with the bond vibration of Cr-O-Cr. The peak characteristic of Cr(III) which occurs around 600 cm−1 was not recorded in this experiment due ...
The equimolar peak strength ratio(K)between carbonyl(1716cm-1 )and mono-substituted benzene ring (699 cm-1 )was calculated by fitting linear relationship between peak area ratio and mole ratio by varying the molar ratio of 3-benzenepropanoic acid and adipic acid.The K is equal to 6.39 in ...
Quality spectra with high peak resolution and smoothness of baseline are a prerequisite for further quantitative analysis. As demonstrated by Faix and Böttcher [40], the traditional KBr pellet method suffers from poor spectral reproducibility caused by various factors including, among others, moisture...