Protein structureProtein–membrane interactionsTransmembrane orientationFourier transform infrared (FTIR) spectroscopy has become one of the major techniques of structural characterization of proteins, peptides, and protein–membrane interactions. While the method does not have the capability of providing the ...
Basic experimental requirements, pros, cons, and means of interpreting the resulting spectra are covered.G.E. TranterEncyclopedia of Spectroscopy and Spectrometry (Third Edition)Tranter, G.E. Protein structure analysis by CD, FTIR, and Raman spectroscopies. In Reference Module in Chemistry, Molecular...
Surface-Modified Poly(methyl methacrylate) Capillary Electrophoresis Microchips for Protein and Peptide Analysis Polymeric materials have emerged as appealing alternatives to conventional inorganic substrates for the fabrication of microscale analytical systems; howev... J Liu,T Pan,AT Woolley,... - 《Anal...
Protein secondary structure analyses from circular dichroism spectroscopy: Methods and reference databases Circular dichroism (CD) spectroscopy has been a valuable method for the analysis of protein secondary structures for many years. With the advent of synchro... L Whitmore,BA Wallace - 《Biopolymers...
Fourier transform infrared (FTIR) spectroscopy is an experimental technique used initially for qualitative and quantitative analysis of organic compounds, providing specific information on molecular structure, chemical bonding and molecular environment. For many years, FTIR has been successfully employed for ...
Structure determination of multidomain proteins or protein–membrane complexes is one of the most challenging tasks in modern structural biology. High-resolution techniques, like NMR or X-ray crystall...
2D FTIR gives new information about the pressure effect on the structure and dynamics of macromolecular systems. Application of this analysis to proteins c... L Smeller,P Rubens,J Frank,... - 《Vibrational Spectroscopy》 被引量: 25发表: 2000年 Interaction between bacterial cell membranes and na...
Correlation Study of PVDF Membrane Morphology with Protein Adsorption: Quantitative Analysis by FTIR/ATR Technique Microporous PVDF membranes were used as protein capture matrices in immunoassays.Because the most common labels in immunoassays were detected based on the colour change,an understanding of how...
Analysis of biological samples like proteins, lipids, tissues, cells, and various cell extracts can be easily carried out using FTIR spectroscopy. Various types of analysis like protein structure elucidation, protein...
1.2. ATR-FTIR data analysis of secondary structures Prior to curve fitting, a straight baseline passing through the ordinates at 1700 and 1600 cm− 1 is subtracted. To resolve overlapping bands, the spectra are processed using PEAKFIT (Jandel Scientific, San Rafael, CA) software. Second-deriva...