Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13; aldolase A; FBP aldolase; FBPA) is 1 of 10 enzymes that comprise the glycolytic (Embden–Meyerhof–Parnas) pathway. It is one of three tissue-specific isozymes (A, B, C) found in mammals. Type A are found in muscle and red blood cel...
Fructose 1, 6-bisphosphate aldolase [EC 4. 1. 2. 13] from spores of PCI 219 was purified to a nearly homogeneous state; the corresponding enzyme from vegetative cells was also partially purified, and their properties were compared.The molecular weight of the aldolase from spores was similar ...
Fructose-bisphosphate aldolase B ELISA Kit的详细说明书 检测范围: 使用:本试剂仅供科研使用 保存:2-8℃ 有效期:6个月 检测方法:ELISA 产品性状:96T/48T,盒装液体 贮藏条件:2-8°C 产品主要成分:酶标板,试剂,标准品等。 种属:马铃薯、鹿、羊、鸡、鸭、鱼、人、大鼠、小鼠、豚鼠、仓鼠、裸鼠、兔子、猪、犬...
These enzymes, ketohexokinase (fructokinase – EC 2.7.13) aldolase B (fructose-1,6-bisphosphate aldolase – EC 4.1.2.13) and triokinase (EC 2.7.1.28) bring about the metabolic assimilation of exogenous fructose in these organs. Specific Pathway of Fructose Metabolism Newly absorbed fructose in ...
Fructose-2,6-bisphosphate and AMP each acted as mixed inhibitors of FBPase 1 from mussel mantle, whereas the mechanism of inhibition in each case is different. The joint action of Fru-2,6-P2 and AMP is a synergistic inhibition of FBPase ... R Barcia,JI Ramos-Martinez - 《Comparative Bioc...
Fructose-1,6-bisphosphate aldolase. A rat brain S100-binding protein, R40,000, has been isolated, characterized, and identified as fructose-1,6-bisphosphate aldolase. R40,000 was purified by ... DB Zimmer,LJV Eldik - 《Journal of Biological Chemistry》 被引量: 400发表: 1986年 The role ...
6-bisphosphate aldolase family encompass two separate classes of enzymes differing in their enzymatic mechanisms. The class I family employs an active site lysine in Schiff base formation whereas the class II aldolases employ a Zn2+ion as cofactor. Human FBPA belongs to the class I family, ...
(1965) with modifications: 1 ml PFK assay mixture contained 1 mM fructose-6-phosphate, 0.02 mM fructose- 2,6-bisphosphate, 0.2 mM NADH, 1 U aldolase, 5 U triose-3-phosphate isomerase, 5 U glycerol-3-phosphate dehydrogenase, 0.02 mM AMP, 1 mM ATP in the relevant buffer (50 mM bis-...
For instance in glycolysis, fructose 1,6-bisphosphate is reversibly cleaved into the triose dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, whereas in gluconeogenesis, the bisphosphate is formed through action of aldolase (fructose-1,6-bisphosphate or FBP aldolase,1 EC 4.1.2.13). FBP ...
Fructose-1,6-bisphosphatase (FBPase,2 EC 3.1.3.11), a key enzyme of gluconeogenesis, catalyzes the hydrolysis of fructose 1,6-bisphosphate to form fructose 6-phosphate and orthophosphate. It is the reverse of the reaction catalyzed by phosphofructokinase in glycolysis, and the product, fructose 6...