With a gas cromatograph equipped with a flame-ionization detector it was possible to detec: 1 ng of polystryrene, 10 ng of polybutadiene and 100 ng of poly(methyl methacrylate). After the addition of microgram amounts of another substance the yield of the pyrolysis procducts from the ...
In the presence of prostaglandin E1, Mg(2+)-dependent platelet adhesion to collagen was inhibited by avicine pseudocyanide with an IC50 of 278 +/- 16 microM. These data indicate that avicine pseudocyanide is an inhibitor of collagen-induced platelet aggregation and platelet-collagen adhesion....
Binding was of high affinity (Kd = 25 and 19 nM for susceptible and resistant insects, respectively) and the concentration of binding sites was high (Rt = 140 pmol/mg for both). Despite the narrow-spectrum resistance, binding of 125I-labeled Vip3Aa to BBMV of resistant and...
In HG females with a demonstrated history of infertility, progesterone injections (1 mg s.c. daily) beginning on Day 1 postcoitum (p.c.) increased the proportion of animals pregnant at Day 17 of gestation. Twice-daily injections of 100 microgram of ovine prolactin (PRL) in alkaline saline ...
The length distribution of F-actin produced from Mg-polymer formed in the presence of β-actinin was investigated in detail by electron microscopy. When Mg-polymer was formed at a low MgCl 2 concentration (approx. 1 mM), where a fairly large amount of G-actin coexisted with Mg-polymer, an...
MIL-68 (Ga) was subjected to the developed method for the extraction and preconcentration of parabens. Standard solution of derivatized parabens Since the direct injection of the standard solution of parabens results in peak tailing, 10 µL of 5000 mg L−1 stock solution (with respect to ...
One unit of catechol 2,3- dioxygenase activity is defined as the amount of enzyme needed to convert 1 μmol of catechol to 2-hydroxymuconic semialdehyde in 1 minute per mg of protein. Protein concentration was determined using the Bradford method [38]. Purification of His6-tagged MobC ...
Actin activated the Mg ++ ATPase activity by an average factor of 6. No cofactor protein was required for this activity. These properties are similar to those of myosin isolated from Starfish eggs, Physarum, and Dictyostelium. Carnivorous amoeba myosin bound rabbit muscle F actin in the absence ...
MG132 to inhibit the proteasome.jWestern blot analysis of ubiquitinated AGO2 immunoprecipitated from NCI-N87 cells with or without MIR200CHG knockdown. The cells were treated with MG132 to inhibit the proteasome.kThe schematic illustration of the mechanism by which MIR200CHG stabilized miR-200c ...
HanSD and TGR rats were at the age of eight weeks anesthetized with an intraperitoneal injection of ketamine/midazolam mixture (Calypsol, Gedeon Richter, Hungary, 160 mg/kg and Dormicum, Roche, France, 160 mg/kg). Volume overload-induced heart failure was then induced by creating a shunt be...