Associate professor of pharmaceutical sciencesDr. Rocco Rotello, professor of zoologyDr. Mike Mendeland associate professor of biologyDr. Robert Parishave been researching freeze-tolerant frogs native to Ohio. These frogs are unique because they completely freeze during winter hibernation....
To amplify DNA of the 18S rRNA gene of Perkinsea-like organisms within NAG01, primers 86F-B, 300F-B, 1294 R, and 1282 R were used7. Polymerase chain reaction was carried out using GoTaq® Flexi DNA polymerase (Promega Corporation, Madison, Wisconsin, USA). Each 25-µl ...