AkhilSrivastava, ...RajagopalRamesh, inSeminars in Cancer Biology, 2022 7.3Freeze-thaw Freeze-thaw is a conventional method of cellular transfection and loading of drugs inliposomes[159]. This method involves incubation ofexosomesand drugs at room temperature and at below freezing temperatures (−80...
2) rapid frozen thaw method 快速冻融法 1. In order to obtain total DNA from Magnaporthe grisea for the study of molecular biology in a rapid, high efficiency and economic way, the authors have developed a new method——rapid frozen thaw method. 为了快速、简便和经济地获得稻瘟病菌的总...
changes, which in turn could potentially cause protein aggregation. This study provides a systematic approach in understanding and selecting the ideal freeze–thaw conditions for manufacturing of protein-based therapeutics. It identifies the importance of balancing different excipients with an overall goal ...
Whatever method you use, you should always be mindful of the changes you are causing in your samples during freezing and thawing. Especially those that cannot be seen—they could affect your results. Catriona Paul Catriona has a PhD in Reproductive Biology from the University of Edinburgh. ...
PURPOSE: To provide a method for storing erythrocytes without requiring cleaning of the thawed erythrocytes before transfusion by dipping the plural number of erythrocytes into a solution containing a specific concentration of monosaccharides capable of penetrating into a membrane of the erythrocyte and the...
All samples were distilled using the low-blank cold chromium distillation method described in the study by Kallmeyer et al. (2004). Briefly, centrifuged sediment was diluted with 10 ml dimethylformamide 587 The ISME Journal Freeze–thaw effects on bacteria in sediments JE Sawicka et al 588 and ...
freeze–thaw stress is essential for mitigating its adverse effects on yeast performance. This review delves into the intricate mechanisms underlying freeze–thaw stress, focusing specifically onSaccharomyces cerevisiae, the primary yeast used in baking, and presents a wide range of biotechnological ...
Because the fracture plane tends to run between the two membrane leaflets, the method has become an indispensable tool in membrane biology (Severs, 2007). If the replica is treated with sodium dodecyl sulfate (SDS) instead of sodium hypochlorite, molecules cast by the platinum/carbon deposition ...
Freeze-dried storage of decellularized heart valves provides easy storage and transport for clinical use. Freeze-drying without protectants, however, results in a disrupted histoarchitecture after rehydration. In this study, heart valves were incubated i
The activity of six hydrolytic soil extracellular enzymes (α-glucosidase, β-glucosidase, cellobiohydrolase, β-xylosidase, N-acetyl-β-glucosaminidase, acid phosphatase; Table S1) were measured following the 96-wellplate fluorometric method described in Freeman et al. (1995) and Dunn et al. (2014...