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Then, the muscles were placed into a sterile dish with Krebs-Henseleit buffer (KHB) composed of 119 mM NaCl, 4.7 mM KCl, 2.5 mM CaCl2, 1.2 mM MgSO4, 1.2 mM KH2PO4 and 25 mM NaHCO3 (pH 7.4). Raman spectra of the ex vivo gastrocnemius muscle were measured from at least five ...
PROCEDURE: The patient was taken to the operating room where general anesthesia was found to be adequate. She was prepped and draped in normal sterile fashion. The bladder was cleared of 200 cc of urine. Legs were placed in Allen stirrups. A weighted speculum was inserted vaginally and the ...
a) First Flow: 10% off price (time bound to 2 hours) b) Second Flow: 10% off, free 4 pack of universal reusable bags c) Third Flow: 10% off with 2-business day shipping for free and 4 pack of reusable bags Abandoned Visitor for Breg products (excludes power cords, sterile ...
In the next step, 70% ethanol at a 2∶1 volumetric ratio was added to the samples and they were passed through a double-layer sterile gauze and centrifuged for 5 min at 1600 g. The sediments were then preserved in 70% ethanol until use. Taeniid eggs were retrieved from fecal material ...
To prepare the microbiota suspensions, the stools from each group were transferred to an anaerobic hood where they were pooled per group, homogenized 1:4 in saline with 20% sterile glycerol and then filtered through a 70-µm strainer to remove large particles before being aliquoted and frozen...
Each cecum was collected in a sterile plastic bag (Nasco, Saugerties, NY USA) and transported in liquid nitrogen to the laboratory, where it was stored at −80°C and promptly processed for DNA extraction. Salmonella Detection Samples were processed for the detection of Salmonella using the ...
Cell-free DNA was extracted from 1.6 ml of maternal plasma using a QIAamp blood mini kit (Qiagen, Hilden, Germany) as described previously.9 DNA was eluted into 30 ml of sterile and DNase-free water. Sex determination using nested PCR of cff-DNA Sex determination was performed using a ...
The spectra showed consistent vibration peaks in both cases, demonstrating the similarity between the native jellyfish tissue (−Dcell) and the scaffold obtained post-processing (+Dcell), maintaining the characteristic peak of “triple helix” in Amide III, and a lower frequency of Amide A. ...