Since the advent of cfDNA-based non-invasive prenatal testing, myriad applications of cfDNA in diagnostic medicine have been established.17, 18, 19 These short fragments of circulating DNA are the debris of dead cells from across the body. The value of cfDNA as a quantitative marker of tissue...
the maternal origin. Then insert-sizes of the remaining reads were tested under four different hypotheses followed by a median comparison to test whether the alternative allele fragments were indeed statistically different and shorter than the reference allele fragments. To compare the difference of frag...
Chen SY, Wang Y, Telen MJ, Chi JT: The genomic analysis of erythrocyte microRNA expression in sickle cell diseases. PLoS ONE 2008,3(6):e2360. Article PubMed PubMed Central CAS Google Scholar Lee KH, Su YD, Chen SJ, Tseng FG, Lee GB: Microfluidic systems integrated with two-dimension...
Apply the soft transparent graphene microelectrode array forin vivorecording of electrical activity of retinal ganglion cells at single-cell level She also discussed research and other innovations they might consider developing. "Based on nanomaterials and nanotechnology, we seek to develop techniques that...
镰状红细胞贫血症产生的原因是β珠蛋白基因的第6外显子由GTG突变为GAG,从而导致β-珠蛋白第6位氨基酸谷氨酸(Glu)被缬氨酸(Lys)所代替,使得正常的血红蛋白(haemoglobin A,HbA)被镰状血红蛋白(haemoglobin sickle, HbS)所取代。而β-地中海贫血症则由β珠蛋白基因内多样化的点突变或者该基因部分片段缺失所造成。在...
However, such approaches require rigorous laboratory testing to ensure the viability and minimize the antagonism among strains during fermentation, aiming to simulate synergistic interactions rather than inhibition [53]. Once the inoculants are prepared and the fermentation conditions optimized on a small ...
7.2. The Screening of Iron Phytochelators and Other Chelators in Cell Models of Iron Metabolism Different in vitro and in vivo studies were carried out to identify the biological, toxicological and pharmacological properties of phytochelators and also of other chelators in a screening procedure ...
镰状细胞病(sickle cell disease, SCD)是由于β-珠蛋白基因的第7个密码子的单基因点突变造成的。Hoban等[44]利用ZFNs特异性靶向于β-珠蛋白基因,并诱导CD34+造血干细胞和祖细胞中的DNA被切割。当ZFNs与整合酶缺陷型慢病毒载体或寡核苷酸供体一起传送进细胞时,有效地实现了β-珠蛋白基因座的基因矫正。Hoban的研究...
It was there, at a sun-drenched party benefiting squamous-cell research, that Katherine met her first husband. His name was Huff Cornbright, of the antifreeze and real-estate Cornbrights, and he proposed on their third date. They were married on Gibson Beach at Sagaponack, Long Island, wher...
and candle-lit hours in Peter’s bedchambershe knows the peril of her position. Peter’s attentions are fickle and his rages powerful; his first wife is condemned to a prison cell, her lover impaled alive in Red Square. And now Catherine faces the ultimate test: can she keep the Tsar...