each sample was then converted accordingly. The copy number of the PCR product was calculated first by dividing the mass by its molecular weight, calculated based on its unique sequence (http://www.bioinformatics.org/sms2/dna_mw.html), and multiplied by Avogadro’s constant. The final values ...
(Beckman Coulter, Indianapolis, IN) and eluted in 21.5 µl RT-PCR grade water (Thermo-Fisher, Waltham, MA). Restriction digestion was done with 2.5 µl 10xCutSmart buffer and 1 µl MspI (NEB) for 18 h at 37 oC and 20 min at 65oC. 0.5 µl 10xCutSmart ...
Christie, from Indianapolis, was having a crisis about just turning 40, Bibika from Estonia was wondering if it's true that it rains for 10 months of the year in England, and a second German, Biom, was disappointed about returning to the office after his summer break and was hoping his...
PTS Public Test Server (gaming) PTS Permission to Speak PTS Phosphotransferase PTS Phone to Show (real estate) PTS Partido de los Trabajadores Socialistas (Spanish: Socialist Workers Party; Argentina) PTS Polymer Technology Systems (diagnostic tests; Indianapolis, IN) PTS Post- och Telestyrelsen (...
Das 650 pb PCR-Produkt wurde durch Gelektrophorese isoliert und mit pCR1000 (Stratagene Cloning Systems) ligiert. Das sich ergebende Plasmid wurde dazu verwendet, um E. coli XL-1 Zellen zu transformieren. Die Plasmid-DNA wurde von einer ausgewählten Transformante, bezeichnet als G13/pCR...
(see Results) was subcloned into a plasmid vector prior to sequence analysis. The five kb intron 43 was amplified using a commercially available kit for long PCR (Boehringer-Mannheim, Indianapolis, Ind.). The cycling times and temperatures were as follows: initial denaturation, 93° C., 2 ...
Attorney, Agent or Firm: S&F/WEHRW (Indianapolis, INDIANA, US) Claims: What is claimed: 1. A method for detecting an analyte in a sample suspected of containing the analyte, the method comprising: (a) providing a conjugate substrate complex, wherein the conjugate substrate complex comprises ...
PCR Primer Type I β3 Secreted Protein NDF IgG Sp EGF β 3 Spermatogonia Tubular Somatic Cells 100 Spermatocytes Interstitial Somatic Cells 75 Type I β2a Transmembrane Protein NDF IgG Sp EGF β 2 TM Type I α2b Transmembrane Protein NDF IgG Sp EGF α 2 TM 50 25 0 1 2 3 4 CSPG5 ...
Cells were then collected and measured by flow cytom- etry (Epics-XL, Beckman, Indianapolis, IN, USA). Additionally, cells were also stained by Hoechst and Lysotracker Red according to the manufacturer's instructions, and fluo- rescent images were recorded using a fluorescence microscope (EVOS...
PCR is a technique for making many copies of a specific template DNA sequence. The reaction consists of multiple amplification cycles and is initiated using a pair of primer sequences that hybridize to the 5′ and 3′ ends of the sequence to be copied. The amplification cycle includes an ini...