polymerase chain reaction Thesaurus Medical Financial Acronyms Encyclopedia Wikipedia n.Abbr.PCR A technique for amplifying DNA sequences in vitro by separating the DNA into two strands and incubating it with oligonucleotide primers and DNA polymerase. It can amplify a specific sequence of DNA as many...
and in revised form, July 30, 2010 Brenda Thornton and Chhandak Basu† School of Biological Sciences, University of Northern Colorado, Greeley, Colorado 80639 Real-time PCR (quantitative PCR or qPCR) has become the preferred method for validating results obtained from assays which measure gene ...
The invention belongs to the field of molecular diagnosis, and discloses a polymerase chain reaction (PCR)-free gene detection method, which comprises the following steps of: forming a sandwich complex by utilizing nanometer probes modified with recognition molecules and encoded molecules, capture ...
Typically, polymerase chain reaction (PCR) products are generated with SITS downstream of the T7 promoter and upstream of the start codon ATG [66]. The 3′ hairpin region of the SITS increases the residence time of the preinitiation complex in the vicinity of the start codon [66]. Using L...
To test the parity detection logic, the N2HET parity RAM has to be made accessible to the CPU in order to allow a diagnostic program to insert parity errors. The control register bit HETPCR.TEST must be set in order to make the parity RAM accessible. ...
Quantification of mRNAs using real-time polymerase chain reaction (PCR) by monitoring the product formation with the fluorescent dye SYBR Green I is being extensively used in neurosciences, developmental biology, and medical diagnostics. Most PCR data analysis procedures assume that the PCR efficiency ...
If a postnatal sample for CMA confirmation was not available, results from pre- or postnatal clinical testing with karyotype, quantitative fluorescent polymerase chain reaction (QF-PCR), fluorescence in situ hybridization (FISH), or CMA were used for genetic confirmation, if available. The cases wit...
aCell-free fetal DNA in maternal plasma presents interest as it can possibly serve as a pregnancy associated biomarker. For its quantitation, we investigate the use of fluorescent polymerase chain reaction (PCR) amplification of short tandem repeats (STRs) in pregnancies irrespective of sex. 当它...
the buffers from the MagMeDIP kit were added, as per the manufacturer’s instructions. The mixture was heated to 95 °C for 10 min, then immediately placed on ice for 10 mins. Each sample was partitioned into two 0.2 ml PCR tubes: one for 10% input control (7.9 µl...
Quantitative real-time polymerase chain reaction (RT-PCR) The cartilage-related gene expression in all groups was investigated by RT-PCR. The joint cartilage samples (all the cartilage on the distal femur) were cut into fine pieces and homogenized after 8 weeks of treatment. The same amount of...