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Briefly, the cell pellet was resuspended in 3× packed cell volume in buffer I: 20 mM, pH 7.8, HEPES-KOH, 100 mM KCl, 1.5 mM MgCl2, 0.2 mM EDTA, 20% glycerol, 0.5% NP-40, 1 mM DTT, 1× complete protease inhibitor, and 1x phosphatase-inhibitor cocktails I and II (Sigma-Aldrich...