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Two separate populations of U2OS cells were maintained and fed either heavy isotope containing lysine or normal lysine (Thermo Pierce, Rockford, IL, USA). Cells were passaged seven times to fully incorporate the labeled amino acids. Cells were then lysed with a buffer containing the following: ...
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Extracellular release of bacterial outer membrane proteins has been suggested to mainly occur via outer membrane vesicles. This study investigated the presence and conservation of peptidoglycan-associated lipoprotein (AaPAL) among A. actinomycetemcomitans strains, the immunostimulatory effect of AaPAL, ...
Heparin is a widely used anticoagulant which inhibits factor Xa and thrombin through potentiation of antithrombin. We recently identified that the nucleic acid stain SYTOX reacts with platelet polyphosphate due to molecular similarities, some of which are shared by heparin. We attempted to study hepari...
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