1). The foundational principle is that we can construct RiPP biosynthetic pathways totally in vitro using CFE to co-express the precursor peptides and modification enzymes to form mature RiPPs. As a model, we chose to develop UniBioCat with lanthipeptides, which are members of the rapidly ...
1). Our approach relied on cell-free DNA-assembly18 and CFE37 to build site-saturated, sequence-defined protein libraries. This workflow had five steps: (i) a DNA primer containing a nucleotide mismatch introduces a desired mutation through PCR, (ii) DpnI digests the parent plasmid, (iii)...
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3.2.4. Virus-Like Particles Virus-like particles (VLPs) are capsids of viruses lacking genomic material, meaning that they are a highly organized and symmetrical aggregations of proteins, capable of carrying molecules of interest within them. As such, production of VLPs allows for study of viral...
Finally, it should be highlighted that the presented methodology could be used to deal with some typical aerospace applications which require an efficient tool to analyze composite structures characterized by complex geometries, with different material and thickness distributions. Acknowledgments: The ...
1.6.5.2. The CP/command center is not responsible for creating, funding or maintaining CAT/ EOC facilities or CAT support material (books, checklists, slides, equipment, etc.). 1.6.6. The CP will not function as a primary or alternate base armory. This stipulation does not pre- clude ...
Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much attention for biomanufacturing and synthetic biology. However, activating membrane-dependent functionality of cell-derived vesicles in bacterial CFE systems has been limited. Here, we address this limitation by charact...
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Keywords: reactive vapor deposition; hydrophobic; Janus material; absorbant; separation 1. Introduction Industrial water contamination is a pernicious worldwide problem that wreaks tremendous, long-lasting environmental damage and particularly cripples developing economies. Numerous research efforts are ...
Such a material would allow not only the convenient extrusion of electrolytes but would also serve as a material for printing flexible batteries in 3D printers. However, no thermoplastic solid electrolyte offers the optimum balance between safety, ionic conductivity, and mechanical stability. Recently,...