BSA (0.2% w/v) acetic acid (0.01% v/v) solution to have 10x of the highest concentration to be tested. In 96-well PCR plates (Axygen, #PCR-96-SG-C) two-fold serial dilutions were made from columns 1–10 in each row specified to each peptide and BSA (0.2% w/v) acetic acid ...
AlphaLISA reactions were carried out in 50 mM HEPES pH 7.4, 150 mM NaCl, 1 mg/mL BSA, and 0.00015 v/v TritonX-100 (hereafter referred to as Alpha buffer). All components were dispensed using an Echo 525 liquid handler from a 384-Well Polypropylene 2.0 Plus microplate (Labcyte...
A solution of 5 μg/mL FGF2 (recombinant human FGF-basic, Peprotech, New Jersey, USA) containing 0.1 % BSA (albumin fraction V (Carl Roth GmbH + Co. KG) was prepared in PBS. The sterile films (12 mm diameter discs) were incubated in 300 μL FGF2 solution overnight (18 h was ...
Methanol fixed cells were washed three times in PBS and blocked in 1% (w/v) BSA in PBS for 10 min. Primary antibody (diluted in BSA block) was added for 1 h at room temperature. Coverslips were washed three times in BSA block and then fluorophore-conjugated secondary antibody (diluted ...
To earn this Adventure, Bear Cub Scouts may enroll in and complete a swimming instruction program offered by an accredited instructorORpass the BSA swimmer testORcomplete the requirements below. With your den or an adult, identify the attributes of qualified adult supervision at a ...
We also evaluated LINA’s dependence on the number of planes of the input by training new pixel-regression models using QPI inputs with one, two, and four planes, respectively. Supplementary Fig.7aquantifies and compares the performance of the eight-plane model to these three new models. We...
The resulting supernatant was then centrifuged at 9000g for 15 min at 4 °C and resuspended in FFA-free HES buffer with 0.2% BSA. A small aliquot of purified mitochondria was reduced after the addition of a small amount of sodium dithionate (SDT, 10 mM, Sigma–Aldrich, MO, USA) to ...
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m2ultrafiltration module. Six retentate volumes of buffer S30B were run against the lysate at 4 °C. After diafiltration, the retentate was centrifuged for 30 min at 12,000 × gat 4 °C. The protein concentration of the retentate was quantified using a Bradford BSA Protein ...
At each passage, cells were harvested, washed with PBS and incubated at 4 °C for 1 h in 50 μl of FC buffer (0.01% sodium azide/0.5% BSA in PBS) with specific concentrations of primary antibodies (SI, Table 2). When necessary, this was followed by a wash with PBS and ...