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each 10-cm segment of the small intestine was cut in the middle and the resulting 20 specimens were dissected with scissors in safe-lock tubes (2.0 mL; Eppendorf, Hamburg, Germany) containing 1 mL cold (4 ◦C) homogenization buffer on ice (140 mM NaCl, 20 mM HEPES, 1 tablet protease...
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