原间隔邻近基序(PAM:protospacer adjacent motif),CRISPR关联抗病毒复合物(Cascade:CRISPR-associated complex for antiviral defense),trans-activating CRISPR RNA (tracrRNA),多核苷酸磷酸化酶(PNPase:polynucleotide phosphorylase)。Cse1为Ecoli亚类Cas;Csy1-4为Ypest亚类Cas;Csn2为Nmeni亚类Cas;Csm3为Mtube亚类Ca...
Our artificial chimera couples a protein segregation and unfolding process to a downstream proteolytic reaction, resulting in a semisynthetic prototype of the 26S proteasome: a self-compartmentalized and unfoldase-assisted protein degradation machine3. Through precise control of stoichiometry, spatial ...
Similarly, EGFP with a yield of around 300 µg/mL [66], and an active multisubunit enzyme heterodimeric farnesyl transferase (FTase) [36] were synthesized using the L. tarentolae CFPS [36]. Codon optimization is another important parameter that plays a crucial role in increasing the ...
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(t-PA); bombesin; thrombin; hemopoietic growth factor; tumor necrosis factor-alpha and -beta; enkephalinase; RANTES (regulated on activation normally T-cell expressed and secreted); human macrophage inflammatory protein (MIP-1-alpha); a serum albumin such as human serum albumin; Muellerian-...
coli CFPS through recreation of the biosynthetic pathway and used to convert apo [FeFe] hydrogenase to active protein [132]. 3.2.8. Miscellaneous Applications CFPS has also been used for a number of miscellaneous applications, including studies of translational machinery, genetic circuits, metabolic ...
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Geissler et la., Male pseudohermaphroditism caused by mutations of testicular 17β-hydroxysteroid hehydrogenase 3. Nat. Genetics, 7: 34-9 (1994). Gencic et. al., Conservative amino acid substitution in the myelin proteolipid protein of Jimpymsd mice. J. Neurosci., 10(1):117-24 (1990)...
Q9N0V4 15 Glutathione S-transferase Mu 1 GSTM1 0.035 8.081 Glutathione tranferase activity, cellular detoxification, cellular growth and proliferation Q2KIR8 11 L-threonine 3-dehydrogenase, TDH 0.010 5.275 Co-enzyme binding, oxidoreductase E1BHZ6 19 Glutaminase 2 GLS2 0.007 4.695 Citrulline biosynthes...
Keywords: corrugated pipe; laboratory experiments; numerical study; velocity profile; friction factor; OpenFOAM 1. Introduction Free-surface flow in corrugated pipes is a topic of interest for culvert and drain design. Culverts traditionally are made of large corrugated steel pipes, whose surface is...